ArticlePlant cell reports2016
MsZEP, a novel zeaxanthin epoxidase gene from alfalfa (Medicago sativa), confers drought and salt tolerance in transgenic tobacco.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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39 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.
- Plant responses to high temperature and drought: A bibliometrics analysis.Frontiers in plant science · 2022Pooled it
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- Integrative analyses of metabolome and transcriptome identifies the potential mechanism of Aureobasidium pullulans PA-2 inhibiting Chenopodium album L. growth.BMC plant biology · 2025Article
- Overexpression MsJAR1 gene increased lateral branches and plant height in alfalfa (Medicago sativa L.).Scientific reports · 2025Article
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- Regulation of nitro-oxidative homeostasis: an effective approach to enhance salinity tolerance in plants.Plant cell reports · 2024Review
- Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat.Plant communications · 2023Article
- A Transcription FactorPlants (Basel, Switzerland) · 2023Article
- The role of forkhead-associated (FHA)-domain proteins in plant biology.Plant molecular biology · 2023Review
- Impacts of Radio-Frequency Electromagnetic Field (RF-EMF) on Lettuce (Plants (Basel, Switzerland) · 2023Article
- A nostoxanthin-producing bacterium,Frontiers in microbiology · 2023Article
- Article
- Modifications of Phytohormone Metabolism Aimed at Stimulation of Plant Growth, Improving Their Productivity and Tolerance to Abiotic and Biotic Stress Factors.Plants (Basel, Switzerland) · 2022Review
- Cia Zeaxanthin Biosynthesis,International journal of molecular sciences · 2022Article
- Integrative Comparative Assessment of Cold Acclimation in Evergreen and Deciduous Iris Species.Antioxidants (Basel, Switzerland) · 2022Article
- A Cys2His2 Zinc Finger Transcription FactorFrontiers in plant science · 2022Article
- Comparative Physiological and Transcriptome Profiles Uncover Salt Tolerance Mechanisms in Alfalfa.Frontiers in plant science · 2022Article
- Identification of QTL and candidate genes associated with biomass yield and Feed Quality in response to water deficit in alfalfa (Frontiers in plant science · 2022Article
- Comparative time-course transcriptome analysis of two contrasting alfalfa (Frontiers in plant science · 2022Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses due to its functions in ABA biosynthetic and the xanthophyll cycle. To understand the expression characteristics and the biological functions of ZEP in alfalfa (Medicago sativa), a novel gene, designated as MsZEP (KM044311), was cloned, characterized and overexpressed in Nicotiana tabacum. The open reading frame of MsZEP contains 1992 bp nucleotides and encodes a 663-amino acid polypeptide. Amino acid sequence alignment indicated that deduced MsZEP protein was highly homologous to other plant ZEP sequences. Phylogenetic analysis showed that MsZEP was grouped into a branch with other legume plants. Real-time quantitative PCR revealed that MsZEP gene expression was clearly tissue-specific, and the expression levels were higher in green tissues (leaves and stems) than in roots. MsZEP expression decreased in shoots under drought, cold, heat and ABA treatment, while the expression levels in roots showed different trends. Besides, the results showed that nodules could up-regulate the MsZEP expression under non-stressful conditions and in the earlier stage of different abiotic stress. Heterologous expression of the MsZEP gene in N. tabacum could confer tolerance to drought and salt stress by affecting various physiological pathways, ABA levels and stress-responsive genes expression. Taken together, these results suggested that the MsZEP gene may be involved in alfalfa responses to different abiotic stresses and nodules, and could enhance drought and salt tolerance of transgenic tobacco by heterologous expression.
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