ArticleNucleic acids research2015
The casposon-encoded Cas1 protein from Aciduliprofundum boonei is a DNA integrase that generates target site duplications.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Enrichment of horizontally transferred gene clusters in bacterial extracellular vesicles via non lytic mechanisms.The ISME journal · 2025Article
- Active in vivo translocation of the Methanosarcina mazei Gö1 Casposon.Nucleic acids research · 2023Article
- Casposons - silent heroes of the CRISPR-Cas systems evolutionary history.EXCLI journal · 2023Review
- Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities.Frontiers in microbiology · 2022Article
- Recruitment of Mobile Genetic Elements for Diverse Cellular Functions in Prokaryotes.Frontiers in molecular biosciences · 2022Review
- Sequence specific integration by the family 1 casposase from Candidatus Nitrosopumilus koreensis AR1.Nucleic acids research · 2021Article
- Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9.Bioscience reports · 2021Article
- Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales.Biomolecules · 2020Review
- Structural basis of seamless excision and specific targeting by piggyBac transposase.Nature communications · 2020Article
- Unprecedented Diversity of Unique CRISPR-Cas-Related Systems and Cas1 Homologs in Asgard Archaea.The CRISPR journal · 2020Article
- Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas.eLife · 2020Article
- Sequence motifs recognized by the casposon integrase of Aciduliprofundum boonei.Nucleic acids research · 2019Article
- Integrated mobile genetic elements in Thaumarchaeota.Environmental microbiology · 2019Article
- Origins and evolution of CRISPR-Cas systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.Molecular cell · 2019Article
- History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology.Journal of bacteriology · 2018Review
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- Transposable Element Domestication As an Adaptation to Evolutionary Conflicts.Trends in genetics : TIG · 2017Review
- Mobile Genetic Elements and Evolution of CRISPR-Cas Systems: All the Way There and Back.Genome biology and evolution · 2017Review
- Extreme Deviations from Expected Evolutionary Rates in Archaeal Protein Families.Genome biology and evolution · 2017Article
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2 authors.
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Abstract
Many archaea and bacteria have an adaptive immune system known as CRISPR which allows them to recognize and destroy foreign nucleic acid that they have previously encountered. Two CRISPR-associated proteins, Cas1 and Cas2, are required for the acquisition step of adaptation, in which fragments of foreign DNA are incorporated into the host CRISPR locus. Cas1 genes have also been found scattered in several archaeal and bacterial genomes, unassociated with CRISPR loci or other cas proteins. Rather, they are flanked by nearly identical inverted repeats and enclosed within direct repeats, suggesting that these genetic regions might be mobile elements ('casposons'). To investigate this possibility, we have characterized the in vitro activities of the putative Cas1 transposase ('casposase') from Aciduliprofundum boonei. The purified Cas1 casposase can integrate both short oligonucleotides with inverted repeat sequences and a 2.8 kb excised mini-casposon into target DNA. Casposon integration occurs without target specificity and generates 14-15 basepair target site duplications, consistent with those found in casposon host genomes. Thus, Cas1 casposases carry out similar biochemical reactions as the CRISPR Cas1-Cas2 complex but with opposite substrate specificities: casposases integrate specific sequences into random target sites, whereas CRISPR Cas1-Cas2 integrates essentially random sequences into a specific site in the CRISPR locus.
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