ArticleInternational journal of molecular medicine2016
Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress.
Article in International journal of molecular medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- [Research advances on the mechanism and clinical application of cold atmospheric plasma in promoting wound healing].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2025Review
- A Nonclinical Safety Evaluation of Cold Atmospheric Plasma for Medical Applications: The Role of Genotoxicity and Mutagenicity Studies.Life (Basel, Switzerland) · 2024Article
- Comparing Redox and Intracellular Signalling Responses to Cold Plasma in Wound Healing and Cancer.Current issues in molecular biology · 2024Review
- Cold Argon Athmospheric Plasma for Biomedicine: Biological Effects, Applications and Possibilities.Antioxidants (Basel, Switzerland) · 2022Review
- Some Beneficial Effects of Inert Gases on Blood Oxidative Metabolism:BioMed research international · 2022Article
- Non-Thermal Plasma-A New Green Priming Agent for Plants?International journal of molecular sciences · 2020Review
- Non-thermal dielectric-barrier discharge plasma induces reactive oxygen species by epigenetically modifying the expression of NADPH oxidase family genes in keratinocytes.Redox biology · 2020Article
- Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens.International journal of molecular sciences · 2018Article
- MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation.Scientific reports · 2018Article
- Elucidation of Plasma-induced Chemical Modifications on Glutathione and Glutathione Disulphide.Scientific reports · 2017Article
- Clinical and Biological Principles of Cold Atmospheric Plasma Application in Skin Cancer.Advances in therapy · 2016Review
- Non-thermal Plasma-activated Medium Induces Apoptosis of Aspc1 Cells Through the ROS-dependent Autophagy Pathway.In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to identify the mechanisms through which dielectric-barrier discharge plasma damages human keratinocytes (HaCaT cells) through the induction of oxidative stress. For this purpose, the cells were exposed to surface dielectric-barrier discharge plasma in 70% oxygen and 30% argon. We noted that cell viability was decreased following exposure of the cells to plasma in a time-dependent manner, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The levels of intracellular reactive oxygen species (ROS) were determined using 2',7'-dichlorodihydrofluorescein diacetate and dihydroethidium was used to monitor superoxide anion production. Plasma induced the generation of ROS, including superoxide anions, hydrogen peroxide and hydroxyl radicals. N-acetyl cysteine, which is an antioxidant, prevented the decrease in cell viability caused by exposure to plasma. ROS generated by exposure to plasma resulted in damage to various cellular components, including lipid membrane peroxidation, DNA breaks and protein carbonylation, which was detected by measuring the levels of 8-isoprostane and diphenyl-1-pyrenylphosphine assay, comet assay and protein carbonyl formation. These results suggest that plasma exerts cytotoxic effects by causing oxidative stress-induced damage to cellular components.
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