ArticleScientific reports2015
Producing human ceramide-NS by metabolic engineering using yeast Saccharomyces cerevisiae.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Pioneering microbial synthesis of gangliosides in the filamentous fungus Ashbya gossypii.Biotechnology for biofuels and bioproducts · 2025Article
- Skin-Protective Performance of Alternative Stratum Corneum Formed by a Pseudo-Ceramide-Containing Steroid Lamellar Cream.Experimental dermatology · 2025Article
- Synthetic biology strategies for sustainable bioplastic production by yeasts.Journal of microbiology (Seoul, Korea) · 2025Review
- Harnessing organelle engineering to facilitate biofuels and biochemicals production in yeast.Journal of microbiology (Seoul, Korea) · 2025Review
- Review
- Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides.Synthetic and systems biotechnology · 2025Review
- Engineering of Saccharomyces cerevisiae as a platform strain for microbial production of sphingosine-1-phosphate.Microbial cell factories · 2024Article
- Myostatin Alteration in Pigs Enhances the Deposition of Long-Chain Unsaturated Fatty Acids in Subcutaneous Fat.Foods (Basel, Switzerland) · 2022Article
- Dihydroceramide desaturase promotes the formation of intraluminal vesicles and inhibits autophagy to increase exosome production.iScience · 2021Article
- Review
- Differences in the Fatty Acid Profile, Morphology, and Tetraacetylphytosphingosine-Forming Capability Between Wild-Type and MutantFrontiers in bioengineering and biotechnology · 2021Article
- The Impact of Dietary Sphingolipids on Intestinal Microbiota and Gastrointestinal Immune Homeostasis.Frontiers in immunology · 2021Review
- Eminence of Microbial Products in Cosmetic Industry.Natural products and bioprospecting · 2019Review
- Lipophagy prevents activity-dependent neurodegeneration due to dihydroceramide accumulationEMBO reports · 2017Article
- Structure and conserved function of iso-branched sphingoid bases from the nematodeChemical science · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ceramide is one of the most important intercellular components responsible for the barrier and moisture retention functions of the skin. Because of the risks involved with using products of animal origin and the low productivity of plants, the availability of ceramides is currently limited. In this study, we successfully developed a system that produces sphingosine-containing human ceramide-NS in the yeast Saccharomyces cerevisiae by eliminating the genes for yeast sphingolipid hydroxylases (encoded by SUR2 and SCS7) and introducing the gene for a human sphingolipid desaturase (encoded by DES1). The inactivation of the ceramidase gene YDC1, overexpression of the inositol phosphosphingolipid phospholipase C gene ISC1, and endoplasmic reticulum localization of the DES1 gene product resulted in enhanced production of ceramide-NS. The engineered yeast strains can serve as hosts not only for providing a sustainable source of ceramide-NS but also for developing further systems to produce sphingosine-containing sphingolipids.
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