ArticleBMC complementary and alternative medicine2015
Isolation and characterization of an antifungal compound 5-hydroxy-7,4'-dimethoxyflavone from Combretum zeyheri.
Article in BMC complementary and alternative medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
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- Exploration of Baicalein-Core Derivatives as Potent Antifungal Agents: SAR and Mechanism Insights.Molecules (Basel, Switzerland) · 2023Article
- Fractionation and Antibacterial Evaluation of the Surface Compounds from the Leaves ofTheScientificWorldJournal · 2023Article
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- Antifungal Activity of Extracts, Fractions, and Constituents fromPharmaceuticals (Basel, Switzerland) · 2021Article
- Antifungal Activity of Isolated Compounds from the Leaves ofMolecules (Basel, Switzerland) · 2021Article
- Antibacterial activity of Hibiscus rosa-sinensis L. red flower against antibiotic-resistant strains of Helicobacter pylori and identification of the flower constituents.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2021Article
- An Efflux Pumps Inhibitor Significantly Improved the Antibacterial Activity of Botanicals fromTheScientificWorldJournal · 2021Article
- Effective Inhibition of Candidiasis Using an Eco-Friendly Leaf Extract ofNanomaterials (Basel, Switzerland) · 2020Article
- Anti-Fungal Efficacy and Mechanisms of Flavonoids.Antibiotics (Basel, Switzerland) · 2020Review
- Isolation and Purification of Potent Growth Inhibitors fromMolecules (Basel, Switzerland) · 2018Article
- A complex game of hide and seek: the search for new antifungals.MedChemComm · 2016Article
- In vitro toxicity determination of antifungal constituents from Combretum zeyheri.BMC complementary and alternative medicine · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCombretum zeyheri, belongs to the family Combretaceae and is one of the most popular herbal plants in tropical and subtropical countries. The leaves of Combretum zeyheri have been used as herbal medicine and have been reported to have pharmacological activity which includes anti-bacterial, anti-fungal, anticancer and antioxidant properties. The goal of this study was to isolate, identify and characterize compounds from C. zeyheri leaves which are responsible for its antifungal activity.
methodsThe preliminary isolation of C. zeyheri active compounds was carried out using chromatographic techniques which include sephadex gel column chromatography, silica gel column chromatography and thin-layer chromatography (TLC). The isolated compounds were then investigated for their antifungal activity using broth dilution assay. The combined effect of the most potent compound and an antifungal drug miconazole was investigated using the checkerboard assay. Time-kill assays were conducted for the combinations using the colony counting method. The mechanism of action of 5-hydroxy-7,4'-dimethoxyflavone as a potent antifungal agent was investigated by determining its inhibitory activity on Candida albicans drug efflux pumps using the ciprofloxacin assay. The ability of 5-hydroxy-7,4'-dimethoxyflavone to inhibit antioxidant enzymes as well as the biosynthesis of ergosterol were also investigated.
resultsA total of four pure compounds (A-D) were isolated from C. zeyheri leaf extract. Compound B (5-hydroxy-7,4'-dimethoxyflavone) was found to be active against Candida albicans using broth dilution method. This compound was also found to have synergistic activity on growth of C. albicans when combined with miconazole, completely inhibiting growth after only 4 hrs of incubation. Analysis of ergosterol content from Candida albicans showed a time-dependent decrease to 91 % and 63 % at 16 and 24 hrs respectively, in cells treated with ½ MIC of 5-hydroxy-7,4'-dimethoxyflavone. The compound 5-hydroxy-7,4'-dimethoxyflavone also showed inhibition of both the drug efflux pumps (with IC50 = 51.64 μg/ml) and the antioxidant enzymes (at 5 μM).
conclusionThe compound 5-hydroxy-7,4'-dimethoxyflavone may be partly responsible for the reported antifungal activity of C. zeyheri, and may serve as a potential source of lead compounds that can be developed as antifungal phytomedicines.
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