ArticleCellular microbiology2016
Lysosome biogenesis/scattering increases host cell susceptibility to invasion by Trypanosoma cruzi metacyclic forms and resistance to tissue culture trypomastigotes.
Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 53 citations in OpenAlex.
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- Influence of Topography and Composition of Commercial Titanium Dental Implants on Cell Adhesion of Human Gingiva-Derived Mesenchymal Stem Cells: An In Vitro Study.International journal of molecular sciences · 2023Article
- Transcriptional Signatures and Network-Based Approaches Identified Master Regulators Transcription Factors Involved in Experimental Periodontitis Pathogenesis.International journal of molecular sciences · 2023Article
- Autophagy in protists and their hosts: When, how and why?Autophagy reports · 2023Article
- Depletion of NaPathogens (Basel, Switzerland) · 2022Article
- Gp35/50 mucin molecules of Trypanosoma cruzi metacyclic forms that mediate host cell invasion interact with annexin A2.PLoS neglected tropical diseases · 2022Article
- Successful invasion of Trypanosoma cruzi trypomastigotes is dependent on host cell actin cytoskeleton.The Journal of eukaryotic microbiology · 2022Article
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- The host mTOR pathway and parasitic diseases pathogenesis.Parasitology research · 2021Review
- Shedding ofFrontiers in cellular and infection microbiology · 2021Article
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- All Roads Lead to Cytosol:Frontiers in cellular and infection microbiology · 2021Review
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- The Interplay of Host Lysosomes and Intracellular Pathogens.Frontiers in cellular and infection microbiology · 2020Review
- Autophagy: A necessary process during theVirulence · 2019Review
- Plasmodium Secretion Induces Hepatocyte Lysosome Exocytosis and Promotes Parasite Entry.iScience · 2019Article
- Protein quality control machinery in intracellular protozoan parasites: hopes and challenges for therapeutic targeting.Cell stress & chaperones · 2019Review
- Signal peptide recognition in Trypanosoma cruzi GP82 adhesin relies on its localization at protein N-terminus.Scientific reports · 2019Article
- Host cell protein LAMP-2 is the receptor for Trypanosoma cruzi surface molecule gp82 that mediates invasion.Cellular microbiology · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fundamental question to be clarified concerning the host cell invasion by Trypanosoma cruzi is whether the insect-borne and mammalian-stage parasites use similar mechanisms for invasion. To address that question, we analysed the cell invasion capacity of metacyclic trypomastigotes (MT) and tissue culture trypomastigotes (TCT) under diverse conditions. Incubation of parasites for 1 h with HeLa cells in nutrient-deprived medium, a condition that triggered lysosome biogenesis and scattering, increased MT invasion and reduced TCT entry into cells. Sucrose-induced lysosome biogenesis increased HeLa cell susceptibility to MT and resistance to TCT. Treatment of cells with rapamycin, which inhibits mammalian target of rapamycin (mTOR), induced perinuclear lysosome accumulation and reduced MT invasion while augmenting TCT invasion. Metacylic trypomastigotes, but not TCT, induced mTOR dephosphorylation and the nuclear translocation of transcription factor EB (TFEB), a mTOR-associated lysosome biogenesis regulator. Lysosome biogenesis/scattering was stimulated upon HeLa cell interaction with MT but not with TCT. Recently, internalized MT, but not TCT, were surrounded by colocalized lysosome marker LAMP2 and mTOR. The recombinant gp82 protein, the MT-specific surface molecule that mediates invasion, induced mTOR dephosphorylation, nuclear TFEB translocation and lysosome biogenesis/scattering. Taken together, our data clearly indicate that MT invasion is mainly lysosome-dependent, whereas TCT entry is predominantly lysosome-independent.
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