ArticleScientific reports2015
Superresolution imaging with optical fluctuation using speckle patterns illumination.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Near-isotropic super-resolution microscopy with axial interference speckle illumination.Nature communications · 2025Article
- Chip-based label-free incoherent super-resolution optical microscopy.Light, science & applications · 2025Article
- Noninvasive megapixel fluorescence microscopy through scattering layers by a virtual incoherent reflection matrix.Science advances · 2024Article
- Hyperbolic material enhanced scattering nanoscopy for label-free super-resolution imaging.Nature communications · 2022Article
- Article
- Wide-Field Super-Resolution Optical Fluctuation Imaging through Dynamic Near-Field Speckle Illumination.Nano letters · 2022Article
- High-Throughput, Label-Free and Slide-Free Histological Imaging by Computational Microscopy and Unsupervised Learning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Metamaterial assisted illumination nanoscopy via random super-resolution speckles.Nature communications · 2021Article
- Computational optical imaging with a photonic lantern.Nature communications · 2020Article
- Moments reconstruction and local dynamic range compression of high order superresolution optical fluctuation imaging.Biomedical optics express · 2019Article
- Scattering Assisted Imaging.Scientific reports · 2019Article
- State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.International journal of molecular sciences · 2018Review
- Structured illumination microscopy with unknown patterns and a statistical prior.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superresolution fluorescence microscopy possesses an important role for the study of processes in biological cells with subdiffraction resolution. Recently, superresolution methods employing the emission properties of fluorophores have rapidly evolved due to their technical simplicity and direct applicability to existing microscopes. However, the application of these methods has been limited to samples labeled with fluorophores that can exhibit intrinsic emission properties at a restricted timescale, especially stochastic blinking. Here, we present a superresolution method that can be performed using general fluorophores, regardless of this intrinsic property. Utilizing speckle patterns illumination, temporal emission fluctuation of fluorophores is induced and controlled, from which a superresolution image can be obtained exploiting its statistical property. Using this method, we demonstrate, theoretically and experimentally, the capability to produce subdiffraction resolution images. A spatial resolution of 500 nm, 300 nm and 140 nm with 0.4, 0.5 and 1.4 NA objective lenses respectively was achieved in various samples with an enhancement factor of 1.6 compared to conventional fluorescence microscopy.
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