ArticlePLoS neglected tropical diseases2015
Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Case Report:Frontiers in medicine · 2026Article
- 4,000-year-old Mycobacterium lepromatosis genomes from Chile reveal long establishment of Hansen's disease in the Americas.Nature ecology & evolution · 2025Article
- Geographically dispersed zoonotic tuberculosis in pre-contact South American human populations.Nature communications · 2022Article
- Host Diversity and Origin of Zoonoses: The Ancient and the New.Animals : an open access journal from MDPI · 2020Review
- Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos.PLoS neglected tropical diseases · 2018Article
- Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo.PLoS neglected tropical diseases · 2018Article
- The Repetitive Element RLEP Is a Highly Specific Target for Detection of Mycobacterium leprae.Journal of clinical microbiology · 2018Article
- Mycobacterium leprae genomes from naturally infected nonhuman primates.PLoS neglected tropical diseases · 2018Article
- Correction: Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs.PLoS neglected tropical diseases · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
Zoonotic pathogens that cause leprosy (Mycobacterium leprae) and tuberculosis (Mycobacterium tuberculosis complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with M. leprae, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect M. leprae as well as MTBC in wild marmosets. All marmosets were negative for M. leprae DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.