Evidence map›Paper›PMID 26569490›Full record

ArticlePloS one2015

Two New Potential Barcodes to Discriminate Dalbergia Species.

Rasika M Bhagwat, Bhushan B Dholakia, Narendra Y Kadoo, M Balasundaran, Vidya S Gupta

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Chromosome-scale genome of Indian rosewood (Frontiers in plant science · 2023
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  6. Article
  7. Development of DNA barcodes for selectedSaudi journal of biological sciences · 2020
    Article
  8. Article
  9. Physiological responses of rosewoodsEcology and evolution · 2020
    Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rasika M BhagwatPlant Molecular Biology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.
Bhushan B DholakiaPlant Molecular Biology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.
Narendra Y KadooPlant Molecular Biology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.
M BalasundaranForest Genetics and Biotechnology Division, Kerala Forest Research Institute, Peechi, Thrissur, Kerala, India.
Vidya S GuptaPlant Molecular Biology Group, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, Maharashtra, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA barcoding enables precise identification of species from analysis of unique DNA sequence of a target gene. The present study was undertaken to develop barcodes for different species of the genus Dalbergia, an economically important timber plant and is widely distributed in the tropics. Ten Dalbergia species selected from the Western Ghats of India were evaluated using three regions in the plastid genome (matK, rbcL, trnH-psbA), a nuclear transcribed spacer (nrITS) and their combinations, in order to discriminate them at species level. Five criteria: (i) inter and intraspecific distances, (ii) Neighbor Joining (NJ) trees, (iii) Best Match (BM) and Best Close Match (BCM), (iv) character based rank test and (v) Wilcoxon signed rank test were used for species discrimination. Among the evaluated loci, rbcL had the highest success rate for amplification and sequencing (97.6%), followed by matK (97.0%), trnH-psbA (94.7%) and nrITS (80.5%). The inter and intraspecific distances, along with Wilcoxon signed rank test, indicated a higher divergence for nrITS. The BM and BCM approaches revealed the highest rate of correct species identification (100%) with matK, matK+rbcL and matK+trnH-psb loci. These three loci, along with nrITS, were further supported by character based identification method. Considering the overall performance of these loci and their ranking with different approaches, we suggest matK and matK+rbcL as the most suitable barcodes to unambiguously differentiate Dalbergia species. These findings will potentially be helpful in delineating the various species of Dalbergia genus, as well as other related genera.

Indexed as

DalbergiaDNA Barcoding, TaxonomicDNA, PlantGenetic LociGenetic VariationGeographyIndiaMolecular Sequence DataNucleotidesSpecies SpecificityDNA, PlantNucleotides

Identifiers

PMID26569490
PMCPMC4646644

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.