Evidence map›Paper›PMID 26569118›Full record

ArticlePloS one2015

NGF Modulates trkANGFR/p75NTR in αSMA-Expressing Conjunctival Fibroblasts from Human Ocular Cicatricial Pemphigoid (OCP).

Alessandra Micera, Barbara Stampachiacchiere, Antonio Di Zazzo, Roberto Sgrulletta, Magdalena Cortes, Eduardo Maria Normando, Alessandro Lambiase, Stefano Bonini

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Tissue Remodeling in Ocular Mucous Membrane Pemphigoid.Investigative ophthalmology & visual science · 2023
    Observational
  3. Article
  4. Observational
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Chinese medical journal · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Alessandra MiceraIRCCS-G.B. Bietti Foundation, Rome, Italy.
Barbara StampachiacchiereIRCCS-G.B. Bietti Foundation, Rome, Italy.
Antonio Di ZazzoDepartment of Ophthalmology, University Campus Bio-Medico, Rome, Italy.
Roberto SgrullettaDepartment of Ophthalmology, University Campus Bio-Medico, Rome, Italy.
Magdalena CortesIRCCS-G.B. Bietti Foundation, Rome, Italy.
Eduardo Maria NormandoGlaucoma & Retinal Degeneration Research Group, Visual Neurosciences, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, United Kingdom.
Alessandro LambiaseOphthalmology, Dept. Organi di senso, University of Rome "Sapienza", Rome, Italy.
Stefano BoniniDepartment of Ophthalmology, University Campus Bio-Medico, Rome, Italy.
Istituti di Ricovero e Cura a Carattere Scientifico · ITUniversità Campus Bio-Medico · ITSapienza University of Rome · ITWestern General Hospital · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIn a previous study, we reported the upregulation of Nerve Growth Factor (NGF) and trkANGFR expression in Ocular Cicatricial Pemphigoid (OCP), an inflammatory and remodeling eye disease. Herein, we hypothesize a potential NGF-driven mechanism on fibroblasts (FBs) during OCP remodeling events. To verify, human derived OCP-FBs were isolated and characterized either at baseline or after NGF exposure. MATERIALS AND

methodsConjunctival biopsies were obtained from 7 patients having OCP and 6 control subjects (cataract surgery). Both conjunctivas and primary FB cultures were characterised for αSMA, NGF and trkANGFR/p75NTR expression. Subcultures were exposed to NGF and evaluated for αSMA, NGF, trkANGFR/p75NTR expression as well as TGFβ1/IL4 release. For analysis, early and advanced subgroups were defined according to clinical parameters.

resultsOCP-conjunctivas showed αSMA-expressing FBs and high NGF levels. Advanced OCP-FBs showed higher αSMA expression associated with higher p75NTR and lower trkANGFR expression, as compared to early counterparts. αSMA expression was in keeping with disease severity and correlated to p75NTR. NGF exposure did not affect trkANGFR levels in early OCP-FBs while decreased both αSMA/p75NTR expression and TGFβ1/IL4 release. These effects were not observed in advanced OCP-FBs.

conclusionsTaken together, these data are suggestive for a NGF/p75NTR task in the potential modulation of OCP fibrosis and encourages further studies to fully understand the underlying mechanism occurring in fibrosis. NGF/p75NTR might be viewed as a potential therapeutic target.

Indexed as

ActinsAgedAged, 80 and overBiopsyConjunctivaFemaleFibroblastsFlow CytometryGene Expression RegulationHumansInflammationInterleukin-4MaleMicroscopy, ConfocalMiddle AgedNerve Growth FactorACTA2 protein, humanActinsInterleukin-4Nerve Growth FactorNerve Tissue ProteinsNGF protein, humanNGFR protein, humanReceptors, Nerve Growth FactorReceptor, trkATransforming Growth Factor beta1

Identifiers

PMID26569118
PMCPMC4646573
OpenAlexW2278956043

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.