ArticlePloS one2015
Assessment of the Utility of Whole Genome Sequencing of Measles Virus in the Characterisation of Outbreaks.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 86 citations in OpenAlex.
- Whole-Genome Sequencing of Measles Virus from 2025 Outbreak, Texas, USA.Emerging infectious diseases · 2026Article
- Genomic and Epidemiologic Insights into Ongoing Measles Outbreak, Israel, 2025-2026.Emerging infectious diseases · 2026Article
- Whole genome sequencing of measles viruses: the benefit for outbreak investigation, The Netherlands, 2013-2014.Virus genes · 2026Article
- Multiple Reassortments, Limited Geographic Spread, and Comparable Pathogenicity of Crimean-Congo Hemorrhagic Fever Viruses in Sivas Province, Türkiye.Journal of medical virology · 2026Article
- Article
- Measles and rubella: From global health challenges to advancements in molecular diagnostics in the elimination era.Molecular therapy. Nucleic acids · 2025Review
- Article
- Occupational Transmission of Measles Despite COVID-19 Precautions.Pathogens (Basel, Switzerland) · 2025Article
- Surveillance and agnostic capture sequencing of samples from individuals with rash-associated illness in Mali indicates regional transmission of measles virus from West and Central Africa.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2024Article
- Article
- What's going on with measles?Journal of virology · 2024Review
- Building Quality Control for Molecular Assays in the Global Measles and Rubella Laboratory Network.Vaccines · 2024Review
- Robust and sensitive amplicon-based whole-genome sequencing assay of respiratory syncytial virus subtype A and B.Microbiology spectrum · 2024Article
- Rapid molecular epidemiology investigations into two recent measles outbreaks in Israel detected from October 2023 to January 2024.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Genomic tools for post-elimination measles molecular epidemiology using Canadian surveillance data from 2018-2020.Frontiers in microbiology · 2024Article
- Article
- Measles virus transmission patterns and public health responses during Operation Allies Welcome: a descriptive epidemiological study.The Lancet. Public health · 2023Article
- Utility of MF-non coding region for measles molecular surveillance during post-elimination phase, Spain, 2017-2020.Frontiers in microbiology · 2023Article
- Maintenance of Measles Elimination Status in the United States for 20 Years Despite Increasing Challenges.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Updates on Measles Incidence and Eradication: Emphasis on the Immunological Aspects of Measles Infection.Medicina (Kaunas, Lithuania) · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMeasles is a highly infectious disease caused by measles virus (MeV). Despite the availability of a safe and cost-effective vaccine, measles is one of the world-leading causes of death in young children. Within Europe, there is a target for eliminating endemic measles in 2015, with molecular epidemiology required on 80% of cases for inclusion/exclusion of outbreak transmission chains. Currently, MeV is genotyped on the basis of a 450 nucleotide region of the nucleoprotein gene (N-450) and the hemagglutinin gene (H). However, this is not sufficiently informative for distinguishing endemic from imported MeV. We have developed an amplicon-based method for obtaining whole genome sequences (WGS) using NGS or Sanger methodologies from cell culture isolates or oral fluid specimens, and have sequenced over 60 samples, including 42 from the 2012 outbreak in the UK.
resultsOverall, NGS coverage was over 90% for approximately 71% of the samples tested. Analysis of 32 WGS excluding 3' and 5' termini (WGS-t) obtained from the outbreak indicates that the single nucleotide difference found between the two major groups of N-450 sequences detected during the outbreak is most likely a result of stochastic viral mutation during endemic transmission rather than of multiple importation events: earlier strains appear to have evolved into two distinct strain clusters in 2013, one containing strains with both outbreak-associated N-450 sequences. Additionally, phylogenetic analysis of each genomic region of MeV for the strains in this study suggests that the most information is acquired from the non-coding region located between the matrix and fusion protein genes (M/F NCR) and the N-450 genotyping sequence, an observation supported by entropy analysis across genotypes.
conclusionsWe suggest that both M/F NCR and WGS-t could be used to complement the information from classical epidemiology and N-450 sequencing to address specific questions in the context of measles elimination.
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