Evidence map›Paper›PMID 26568700›Full record

ArticleArchaea (Vancouver, B.C.)2015

Molecular Analysis of Methanogen Richness in Landfill and Marshland Targeting 16S rDNA Sequences.

Shailendra Yadav, Sharbadeb Kundu, Sankar K Ghosh, S S Maitra

Abstract read
In one paragraph

Article in Archaea (Vancouver, B.C.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Shailendra YadavDepartment of Biotechnology, Assam University, Silchar, Assam 788011, India.
Sharbadeb KunduDepartment of Biotechnology, Assam University, Silchar, Assam 788011, India.
Sankar K GhoshDepartment of Biotechnology, Assam University, Silchar, Assam 788011, India.
S S MaitraSchool of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methanogens, a key contributor in global carbon cycling, methane emission, and alternative energy production, generate methane gas via anaerobic digestion of organic matter. The methane emission potential depends upon methanogenic diversity and activity. Since they are anaerobes and difficult to isolate and culture, their diversity present in the landfill sites of Delhi and marshlands of Southern Assam, India, was analyzed using molecular techniques like 16S rDNA sequencing, DGGE, and qPCR. The sequencing results indicated the presence of methanogens belonging to the seventh order and also the order Methanomicrobiales in the Ghazipur and Bhalsawa landfill sites of Delhi. Sequences, related to the phyla Crenarchaeota (thermophilic) and Thaumarchaeota (mesophilic), were detected from marshland sites of Southern Assam, India. Jaccard analysis of DGGE gel using Gel2K showed three main clusters depending on the number and similarity of band patterns. The copy number analysis of hydrogenotrophic methanogens using qPCR indicates higher abundance in landfill sites of Delhi as compared to the marshlands of Southern Assam. The knowledge about "methanogenic archaea composition" and "abundance" in the contrasting ecosystems like "landfill" and "marshland" may reorient our understanding of the Archaea inhabitants. This study could shed light on the relationship between methane-dynamics and the global warming process.

Indexed as

BiodiversitySoil MicrobiologyWaste Disposal FacilitiesWetlandsAnaerobiosisArchaeaCluster AnalysisDenaturing Gradient Gel ElectrophoresisDNA, ArchaealDNA, RibosomalIndiaMethaneMolecular Sequence DataPhylogenyReal-Time Polymerase Chain ReactionRNA, Ribosomal, 16SDNA, ArchaealDNA, RibosomalMethaneRNA, Ribosomal, 16S

Identifiers

PMID26568700
PMCPMC4623359

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.