ArticleParasites & vectors2015
Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Pooled it
- A Noninvasive Fecal-DNA Detection Method for Sarcoptic Mange in the San Joaquin Kit Fox (Animals : an open access journal from MDPI · 2026Article
- Pathology and parasitology of free-ranging coyotes from Tennessee and South Carolina.PloS one · 2025Article
- Surveillance of Sarcoptic Mange in Iberian Ibexes (Animals : an open access journal from MDPI · 2024Article
- Main mites associated with dermatopathies present in dogs and other members of the Canidae family.Open veterinary journal · 2023Review
- Diseases of Iberian ibex (European journal of wildlife research · 2023Review
- Infestation, histology, and molecular confirmation ofInternational journal for parasitology. Parasites and wildlife · 2022Article
- Laboratory-based diagnosis of scabies: a review of the current status.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2022Review
- Non-Invasive Molecular Survey of Sarcoptic Mange in Wildlife: Diagnostic Performance in Wolf Faecal Samples Evaluated by Multi-Event Capture-Recapture Models.Pathogens (Basel, Switzerland) · 2021Article
- Molecular diagnosis of scabies using a novel probe-based polymerase chain reaction assay targeting high-copy number repetitive sequences in the Sarcoptes scabiei genome.PLoS neglected tropical diseases · 2021Article
- Efficacy of afoxolaner plus milbemycin oxime and afoxolaner alone as treatment for sarcoptic mange in naturally infested dogs.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2020Article
- The Management of Scabies in the 21st Century: Past, Advances and Potentials.Acta dermato-venereologica · 2020Review
- Detection ofJournal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- A review of sarcoptic mange in North American wildlife.International journal for parasitology. Parasites and wildlife · 2019Review
- The public health control of scabies: priorities for research and action.Lancet (London, England) · 2019Review
- Sarcoptic Mange of Fox Origin in Multiple Farm Animals and Scabies in Humans, Switzerland, 2018.Emerging infectious diseases · 2019Article
- Detection and identification ofPeerJ · 2019Article
- Characterizing the growth of Sarcoptes scabiei infrapopulations.Experimental & applied acarology · 2018Article
- A newEcology and evolution · 2018Article
- Sarcoptic mange in wild quichua porcupines (International journal for parasitology. Parasites and wildlife · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mite Sarcoptes scabiei has a known host-range of over 100 mammal species including humans. One of the prime objectives of the Sarcoptes-World Molecular Network (WMN) is to design and develop universal Sarcoptes PCR-based diagnosis methods.
methodsWe describe here for the first time two universal mitochondrial-based diagnosis methods: (i) conventional end-point PCR and (ii) TaqMan real-time PCR. The design of both of these universal diagnosis methods was based on Sarcoptes samples collected from 23 host species in 14 countries.
resultsThese methods, based on skin scrapings, were successfully used to etiologically confirm the diagnosis of different clinical degrees of sarcoptic mange in 48 animals belonging to six species. These universal PCR-based diagnosis methods are highly specific, technically sensitive and simple, and are based on the amplification of 135 bp from the Mitochondrial 16S rDNA. The method based on TaqMan real-time qPCR was more sensitive than the conventional end-point PCR.
conclusionsTwo universal PCR-based diagnosis methods for S. scabiei were successfully designed and applied; one based on conventional end-point PCR and the other on TaqMan real-time PCR. We recommend further testing and the application of these new universal methods worldwide.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.