ArticleIranian biomedical journal2016
Characteristics of Human Endometrial Stem Cells in Tissue and Isolated Cultured Cells: An Immunohistochemical Aspect.
Article in Iranian biomedical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Unraveling the Dynamics of Estrogen and Progesterone Signaling in the Endometrium: An Overview.Cells · 2024Review
- Unique expression patterns of the embryonal stem cell marker SOX2 and hormone receptors suggest the existence of a subpopulation of epithelial stem/progenitor cells in porcine and bovine endometrium.Veterinary medicine and science · 2022Article
- The Role of Endometrial Stem/Progenitor Cells in Recurrent Reproductive Failure.Journal of personalized medicine · 2022Review
- Novel microarchitecture of human endometrial glands: implications in endometrial regeneration and pathologies.Human reproduction update · 2022Review
- Differentiation of endometrial stem cells into insulin-producing cells using signaling molecules and zinc oxide nanoparticles, and three-dimensional culture on nanofibrous scaffolds.Journal of materials science. Materials in medicine · 2019Article
- Human menstrual blood: a renewable and sustainable source of stem cells for regenerative medicine.Stem cell research & therapy · 2018Review
- Endometrial Stem Cell Markers: Current Concepts and Unresolved Questions.International journal of molecular sciences · 2018Review
- Article
- Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell.Veterinary world · 2017Article
- Leukemia inhibitory factor increases the proliferation of human endometrial stromal cells and expression of genes related to pluripotency.International journal of reproductive biomedicine · 2017Article
- Evaluation of two endometriosis models by transplantation of human endometrial tissue fragments and human endometrial mesenchymal cells.International journal of reproductive biomedicine · 2017Article
- The effect of stem cell factor on proliferation of human endometrial CD146(+) cells.International journal of reproductive biomedicine · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was to investigate the percentage of the stem cells population in human endometrial tissue sections and cultured cells at fourth passage.
methodsHuman endometrial specimens were divided into two parts, one part for morphological studies and the other part for in vitro culture. Full thickness of human normal endometrial sections and cultured endometrial cells at fourth passage were analyzed via immunohistochemistry for CD146 and some stemness markers such as Oct4, Nanog, Sox2, and Klf4 and the expression of typical mesenchymal stem cell markers CD90, CD105.
results11.88 ± 1.29% of human endometrial cells whitin tissue sections expressed CD146 marker vs. 28±2.3% of cultured cells, CD90 and CD105 were expressed by functionalis stroma (85±2.4 and 89±3.2%) than basalis stroma (16±1.4 and 17±1.9%), respectively (P<0.05). Oct4 and Nanog-expressing cells comprise 1.43±0.08 and 0.54±0.01% of endometrial stromal cells in endometrial sections vs. 12±3.1% and 8±2.9% of cultured cells, respectively. They reside near the glands in the basal layer of endometrium. Sox2 and Klf4 were not commonly expressed in tissue samples and cultured cells. CD9 and EpCAM were expressed by epithelial cells of the endometrium, rather than by stroma or perivascular cells.
conclusionThe human endometrial stem cells and pluripotency markers may be localized more in basalis layer of endometrium. The immunostaining observations of endometrial cells at fourth passage were correlated with the immunohistochemistry data.
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