ArticleScientific reports2015
Human proximal tubule epithelial cells cultured on hollow fibers: living membranes that actively transport organic cations.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 109 citations in OpenAlex.
- Engineered Red Blood Cell-Derived Extracellular Vesicles With Klotho Peptide Protect the Kidney From Fibrosis.Journal of extracellular biology · 2026Article
- A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport Mechanisms.Kidney360 · 2025Article
- Human and rat renal proximal tubule in vitro models for ADME applications.Archives of toxicology · 2025Review
- A Novel Protocol for Culturing Polarized Proximal Tubular Epithelial Cells from Kidney Biopsies: Enhancing Platforms for Drug Excretion and Nephrotoxicity Studies.Journal of xenobiotics · 2025Article
- Thermoforming for Small Feature Replication in Melt Electrowritten Membranes to Model Kidney Proximal Tubule.Advanced healthcare materials · 2025Article
- Vascular units as advanced living materials for bottom-up engineering of perfusable 3D microvascular networks.Bioactive materials · 2024Article
- Proceedings of a membrane update symposium: advancements, scientific insights, and future trends for dialysis membranes for enhanced clinical outcomes in end stage kidney disease patients.Frontiers in nephrology · 2024Article
- Bioengineered Kidney Tubules Efficiently Clear Uremic Toxins in Experimental Dialysis Conditions.International journal of molecular sciences · 2023Article
- 3D vascularised proximal tubules-on-a-multiplexed chip model for enhanced cell phenotypes.Lab on a chip · 2023Article
- Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule.Communications biology · 2023Article
- Clinical-grade human skin-derived ABCB5+ mesenchymal stromal cells exert anti-apoptotic and anti-inflammatory effectsFrontiers in immunology · 2023Article
- Differentiated kidney tubular cell-derived extracellular vesicles enhance maturation of tubuloids.Journal of nanobiotechnology · 2022Article
- Review
- Article
- Human Cholangiocytes Form a Polarized and Functional Bile Duct on Hollow Fiber Membranes.Frontiers in bioengineering and biotechnology · 2022Article
- Role of extracellular matrix components and structure in new renal modelsFrontiers in physiology · 2022Review
- Microphysiological systems in absorption, distribution, metabolism, and elimination sciences.Clinical and translational science · 2022Review
- Development of a hollow fibre-based renal module for active transport studies.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2021Article
- Renal Biology Driven Macro- and Microscale Design Strategies for Creating an Artificial Proximal Tubule Using Fiber-Based Technologies.ACS biomaterials science & engineering · 2021Review
- Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bioartificial kidney (BAK) aims at improving dialysis by developing 'living membranes' for cells-aided removal of uremic metabolites. Here, unique human conditionally immortalized proximal tubule epithelial cell (ciPTEC) monolayers were cultured on biofunctionalized MicroPES (polyethersulfone) hollow fiber membranes (HFM) and functionally tested using microfluidics. Tight monolayer formation was demonstrated by abundant zonula occludens-1 (ZO-1) protein expression along the tight junctions of matured ciPTEC on HFM. A clear barrier function of the monolayer was confirmed by limited diffusion of FITC-inulin. The activity of the organic cation transporter 2 (OCT2) in ciPTEC was evaluated in real-time using a perfusion system by confocal microscopy using 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP(+)) as a fluorescent substrate. Initial ASP(+) uptake was inhibited by a cationic uremic metabolites mixture and by the histamine H2-receptor antagonist, cimetidine. In conclusion, a 'living membrane' of renal epithelial cells on MicroPES HFM with demonstrated active organic cation transport was successfully established as a first step in BAK engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.