ArticleNature communications2015
Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Long-stranded XNA-cssDNA hybrids for robust data storage.Science advances · 2026Article
- BARseq3: a modular system for integrating spatial multi-omics and cellular barcoding in single cells.bioRxiv : the preprint server for biology · 2026Article
- Fabrication of microcolumn arrays for high-throughput oligonucleotide synthesis using 3D printing.Scientific reports · 2025Article
- Discovery of the widespread site-specific single-stranded nuclease family Ssn.Nature communications · 2025Article
- ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets.Nature protocols · 2024Review
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Folding molecular origami from ribosomal RNA.Journal of nanobiotechnology · 2024Article
- Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assayNational science review · 2024Review
- Single-Cell Imaging of mRNA by Target RNA-Initiated RCA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The 3D Genome in Brain Development: An Exploration of Molecular Mechanisms and Experimental Methods.Neuroscience insights · 2024Review
- Probe-based bacterial single-cell RNA sequencing predicts toxin regulation.Nature microbiology · 2023Article
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- (Tn5-)FISH-based imaging in the era of 3D/spatial genomics.Biophysics reports · 2023Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting.Nanoscale · 2022Article
- Robust, versatile DNA FISH probes for chromosome-specific repeats in Caenorhabditis elegans and Pristionchus pacificus.G3 (Bethesda, Md.) · 2022Article
- RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure.Nature protocols · 2022Review
- Cation-Responsive and Photocleavable Hydrogels from Noncanonical Amphiphilic DNA Nanostructures.Nano letters · 2022Article
- Single antibody detection in a DNA origami nanoantenna.iScience · 2021Article
- Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.Nucleic acids research · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology, genetics and synthetic biology, which all require thousands of these at high quality. Inexpensive chip-synthesized oligonucleotide libraries can contain hundreds of thousands of distinct sequences, however only at sub-femtomole quantities per strand. Here we present a selective oligonucleotide amplification method, based on three rounds of rolling-circle amplification, that produces nanomole amounts of single-stranded oligonucleotides per millilitre reaction. In a multistep one-pot procedure, subsets of hundreds or thousands of single-stranded DNAs with different lengths can selectively be amplified and purified together. These oligonucleotides are used to fold several DNA nanostructures and as primary fluorescence in situ hybridization probes. The amplification cost is lower than other reported methods (typically around US$ 20 per nanomole total oligonucleotides produced) and is dominated by the use of commercial enzymes.
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Registered trials
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