Evidence map›Paper›PMID 26567472›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2016

Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.

Todd A Duncombe, Chi-Chih Kang, Santanu Maity, Toby M Ward, Mark D Pegram, Niren Murthy, Amy E Herr

Abstract readEvaluation Study
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Western Blot Analysis in Ewing Sarcoma.Methods in molecular biology (Clifton, N.J.) · 2027
    Article
  2. Materials today. Bio · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Todd A Duncombe *Department of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.
Chi-Chih Kang *Department of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.
Santanu MaityDepartment of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.
Toby M WardDivision of Oncology, Department of Medicine, Stanford University, Stanford, California, CA 94305, USA.
Mark D PegramDivision of Oncology, Department of Medicine, Stanford University, Stanford, California, CA 94305, USA.
Niren MurthyDepartment of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.
Amy E HerrDepartment of Bioengineering, University of California Berkeley, Berkeley, California, CA 94720, USA.

Funding

Towards High Throughput Proteomics: A Micro/nanofluidic Framework for Blotless WDP2OD007294 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI HERR, AMY ELIZABETH · 2010 to 2013
$2.5M
Microfluidic Western Blotting for Targeted Proteomic Analysis of Single Circulating Tumor CellsR21CA183679 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HERR, AMY ELIZABETH · 2015 to 2017
$634k
NCI NIH HHS CA183679NCI NIH HHS R21 CA183679NIH HHS DP2 OD007294NIH HHS DP2OD007294
6 · The paper itself

Abstract

Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.

Indexed as

HydrogelsBlotting, WesternBreast NeoplasmsCell Line, TumorElectrophoresis, Polyacrylamide GelErb-b2 Receptor Tyrosine KinasesExtracellular Signal-Regulated MAP KinasesGreen Fluorescent ProteinsHumansLightNucleocytoplasmic Transport ProteinsPorositySingle-Cell AnalysisTOR Serine-Threonine KinasesEIF4ENIF1 protein, humanERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesExtracellular Signal-Regulated MAP KinasesGreen Fluorescent ProteinsHydrogelsMTOR protein, humanNucleocytoplasmic Transport ProteinsTOR Serine-Threonine Kinasescancer therapiesgradient gelsgrayscale maskshydrogel pore expansionwestern blots

Identifiers

PMID26567472
PMCPMC4708057

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.