ArticleJournal of cell science2016
Activated microglia cause reversible apoptosis of pheochromocytoma cells, inducing their cell death by phagocytosis.
Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 52 citations in OpenAlex.
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- Investigation of a chondroitin sulfate-based bioactive coating for neural interface applications.Journal of materials chemistry. B · 2024Article
- Integrin restriction by miR-34 protects germline progenitors from cell death during aging.Aging cell · 2024Article
- Non-professional efferocytosis of Salmonella-infected intestinal epithelial cells in the neonatal host.The Journal of experimental medicine · 2024Article
- Cell death by phagocytosis.Nature reviews. Immunology · 2024Review
- The Implications of Microglial Regulation in Neuroplasticity-Dependent Stroke Recovery.Biomolecules · 2023Review
- Syk inhibitors protect against microglia-mediated neuronal loss in culture.Frontiers in aging neuroscience · 2023Article
- LPS-stimulated microglial cells promote ganglion cell death in organotypic cultures of quail embryo retina.Frontiers in cellular neuroscience · 2023Article
- Cytokine Signalling at the Microglial Penta-Partite Synapse.International journal of molecular sciences · 2021Review
- Inflammatory neuronal loss in the substantia nigra induced by systemic lipopolysaccharide is prevented by knockout of the P2YJournal of neuroinflammation · 2021Article
- Co-Culturing Microglia and Cortical Neurons Differentiated from Human Induced Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2021Article
- Assessment of the Effects of Stretch-Injury on Primary Rat Microglia.Molecular neurobiology · 2021Article
- Review
- I'm Infected, Eat Me! Innate Immunity Mediated by Live, Infected Cells Signaling To Be Phagocytosed.Infection and immunity · 2021Review
- Deletion of Socs3 in LysMMolecular neurodegeneration · 2021Article
- Towards Reduction or Substitution of Cytotoxic DMSO in Biobanking of Functional Bioengineered Megakaryocytes.International journal of molecular sciences · 2020Article
- Article
- Impact of inflammation on developing respiratory control networks: rhythm generation, chemoreception and plasticity.Respiratory physiology & neurobiology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Some apoptotic processes, such as phosphatidylserine exposure, are potentially reversible and do not necessarily lead to cell death. However, phosphatidylserine exposure can induce phagocytosis of a cell, resulting in cell death by phagocytosis: phagoptosis. Phagoptosis of neurons by microglia might contribute to neuropathology, whereas phagoptosis of tumour cells by macrophages might limit cancer. Here, we examined the mechanisms by which BV-2 microglia killed co-cultured pheochromocytoma (PC12) cells that were either undifferentiated or differentiated into neuronal cells. We found that microglia activated by lipopolysaccharide rapidly phagocytosed PC12 cells. Activated microglia caused reversible phosphatidylserine exposure on and reversible caspase activation in PC12 cells, and caspase inhibition prevented phosphatidylserine exposur and decreased subsequent phagocytosis. Nitric oxide was necessary and sufficient to induce the reversible phosphatidylserine exposure and phagocytosis. The PC12 cells were not dead at the time they were phagocytised, and inhibition of their phagocytosis left viable cells. Cell loss was inhibited by blocking phagocytosis mediated by phosphatidylserine, MFG-E8, vitronectin receptors or P2Y6 receptors. Thus, activated microglia can induce reversible apoptosis of target cells, which is insufficient to cause apoptotic cell death, but sufficient to induce their phagocytosis and therefore cell death by phagoptosis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.