Evidence map›Paper›PMID 26567114›Full record

ArticleMolecular imaging and biology2016

[(11)C]acetate PET Imaging is not Always Associated with Increased Lipogenesis in Hepatocellular Carcinoma in Mice.

Lei Li, Li Che, Chunmei Wang, Joseph E Blecha, Xiaolei Li, Henry F VanBrocklin, Diego F Calvisi, Michelle Puchowicz, Xin Chen, Youngho Seo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular imaging and biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 10 citations in OpenAlex.

  1. [Molecular imaging and biology · 2017
    Trial
  2. Article
  3. Synthesis and Evaluation ofMolecules (Basel, Switzerland) · 2022
    Article
  4. Article
  5. [Molecular imaging · 2022
    Article
  6. Validation of R-2-[Molecular imaging and biology · 2019
    Article
  7. Article
  8. Review
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Lei LiSchool of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Li CheDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, 94143-0912, USA.
Chunmei WangDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, 94143-0912, USA.
Joseph E BlechaDepartment of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA.
Xiaolei LiDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, 94143-0912, USA.
Henry F VanBrocklinDepartment of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA.
Diego F CalvisiInstitute of Pathology, University of Greifswald, Greifswald, Germany.
Michelle PuchowiczDepartment of Nutrition, Case Western Reserve University, Cleveland, OH, USA.
Xin ChenDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA, 94143-0912, USA. xin.chen@ucsf.edu.
Youngho SeoDepartment of Radiology and Biomedical Imaging, University of California, San Francisco, CA, USA. youngho.seo@ucsf.edu.
University of California, San Francisco · USCase Western Reserve University · USHuazhong University of Science and Technology · CNUniversität Greifswald · DE

Funding

Translational InformaticsP30CA082103 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Jennifer Leigh Clarke · 1999 to 2026
$209.7M
UCSF Liver Core CenterP30DK026743 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Holger Willenbring · 1986 to 2026
$30.7M
Mouse Metabolic Phenotyping CenterU24DK076174 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI BRUNENGRABER, HENRI · 2011 to 2015
$2.7M
Molecular Genetics of Liver CancersR01CA136606 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHEN, XIN · 2009 to 2016
$2.6M
NCI NIH HHS P30 CA082103NCI NIH HHS R01 CA136606NIDDK NIH HHS P30 DK026743NIDDK NIH HHS U24 DK076174
6 · The paper itself

Abstract

purposeAltered metabolism, including increased glycolysis and de novo lipogenesis, is one of the hallmarks of cancer. Radiolabeled nutrients, including glucose and acetate, are extensively used for the detection of various tumors, including hepatocellular carcinomas (HCCs). High signal of [(11)C]acetate positron emission tomography (PET) in tumors is often considered to be associated with increased expression of fatty acid synthase (FASN) and increased de novo lipogenesis in tumor tissues. Defining a subset of tumors with increased [(11)C]acetate PET signal and thus increased lipogenesis was suggested to help select a group of patients, who may benefit from lipogenesis-targeting therapies. PROCEDURES: To investigate whether [(11)C]acetate PET imaging is truly associated with increased de novo lipogenesis along with hepatocarcinogenesis, we performed [(11)C]acetate PET imaging in wild-type mice as well as two mouse HCC models, induced by myrAKT/Ras(V12) (AKT/Ras) and PIK3CA(1047R)/c-Met (PI3K/Met) oncogene combinations. In addition, we analyzed FASN expression and de novo lipogenesis rate in these mouse liver tissues.

resultsWe found that while HCCs induced by AKT/Ras co-expression showed high levels of [(11)C]acetate PET signal compared to normal liver, HCCs induced by PI3K/Met overexpression did not. Intriguingly, elevated FASN expression and increased de novo lipogenesis rate were observed in both AKT/Ras and PI3K/Met HCCs.

conclusionAltogether, our study suggests that [(11)C]acetate PET imaging can be a useful tool for imaging of a subset of HCCs. However, at molecular level, the increased [(11)C]acetate PET imaging is not always associated with increased FASN expression or de novo lipogenesis.

Indexed as

LipogenesisAcetatesAnimalsCarbon RadioisotopesCarcinoma, HepatocellularCholesterolLipid DropletsLiver NeoplasmsMicePhosphatidylinositol 3-KinasesPositron-Emission TomographyProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-metras ProteinsTriglyceridesXenograft Model Antitumor AssaysAcetatesCarbon RadioisotopesCholesterolPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-metras ProteinsTriglycerides[11C]acetateDe novo lipogenesisFatty acid synthase expressionHepatocellular carcinomaPET

Identifiers

PMID26567114
PMCPMC4866912
OpenAlexW2202178487

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.