ArticleG3 (Bethesda, Md.)2015
Identification of a Classical Mutant in the Industrial Host Aspergillus niger by Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 77 citations in OpenAlex.
- Article
- The global regulator LaeA controls expression of genes involved in diverse primary and secondary metabolites biosynthesis and their secretion in fungi.Frontiers in microbiology · 2026Review
- Synthesis and antimicrobial evaluation of a new hybrid bis-cyanoacrylamide-based-piperazine containing sulphamethoxazole moiety against rheumatoid arthritis-associated pathogens.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Engineering of Global Transcriptional Regulators (GTRs) inJournal of fungi (Basel, Switzerland) · 2025Review
- Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism inmSphere · 2025Article
- Involvement of LaeA and Velvet Proteins in Regulating the Production of Mycotoxins and Other Fungal Secondary Metabolites.Journal of fungi (Basel, Switzerland) · 2024Review
- Expression of microbial lipase in filamentous fungus3 Biotech · 2024Review
- Citric Acid: Properties, Microbial Production, and Applications in Industries.Molecules (Basel, Switzerland) · 2023Review
- Functional characterization of fungal lytic polysaccharide monooxygenases for cellulose surface oxidation.Biotechnology for biofuels and bioproducts · 2023Article
- AfLaeA, a Global Regulator of Mycelial Growth, Chlamydospore Production, Pathogenicity, Secondary Metabolism, and Energy Metabolism in the Nematode-Trapping FungusMicrobiology spectrum · 2023Article
- Rapid and robust squashed spore/colony PCR of industrially important fungi.Fungal biology and biotechnology · 2023Article
- A newly constructed Agrobacterium-mediated transformation system based on the hisB auxotrophic marker for genetic manipulation in Aspergillus niger.Archives of microbiology · 2023Article
- The putative methyltransferase LaeA regulates mycelium growth and cellulase production in Myceliophthora thermophila.Biotechnology for biofuels and bioproducts · 2023Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Manganese(II) ions suppress the transcription of the citrate exporter encoding geneFrontiers in bioengineering and biotechnology · 2023Article
- Itaconic acid production is regulated by LaeA inMetabolic engineering communications · 2022Article
- Use of Bulk Segregant Analysis for Determining the Genetic Basis of Azole Resistance in the Opportunistic PathogenFrontiers in cellular and infection microbiology · 2022Article
- Microbial Biosynthesis of L-Malic Acid and Related Metabolic Engineering Strategies: Advances and Prospects.Frontiers in bioengineering and biotechnology · 2021Review
- Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development inFrontiers in genetics · 2021Article
- Deletion of theMicroorganisms · 2020Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically characterized a UV-generated A. niger mutant that was originally isolated as a nonacidifying mutant, which is a desirable trait for industrial enzyme production. Physiological analysis showed that this mutant did not secrete large amounts of citric acid and oxalic acid, thus explaining the nonacidifying phenotype. As traditional complementation approaches to characterize the mutant genotype were unsuccessful, we used bulk segregant analysis in combination with high-throughput genome sequencing to identify the mutation responsible for the nonacidifying phenotype. Since A. niger has no sexual cycle, parasexual genetics was used to generate haploid segregants derived from diploids by loss of whole chromosomes. We found that the nonacidifying phenotype was caused by a point mutation in the laeA gene. LaeA encodes a putative methyltransferase-domain protein, which we show here to be required for citric acid production in an A. niger lab strain (N402) and in other citric acid production strains. The unexpected link between LaeA and citric acid production could provide new insights into the transcriptional control mechanisms related to citric acid production in A. niger. Interestingly, the secondary metabolite profile of a ΔlaeA strain differed from the wild-type strain, showing both decreased and increased metabolite levels, indicating that LaeA is also involved in regulating the production of secondary metabolites. Finally, we show that our systems genetics approach is a powerful tool to identify trait mutations.
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