ArticleJournal of bacteriology1989
Specific localization of the lysis protein of bacteriophage MS2 in membrane adhesion sites of Escherichia coli.
Article in Journal of bacteriology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Physiological characterization of single-gene lysis proteins.Journal of bacteriology · 2024Article
- Physiological characterization of single gene lysis proteins.bioRxiv : the preprint server for biology · 2023Article
- Article
- Mechanisms of interactions between bacteria and bacteriophage mediate by quorum sensing systems.Applied microbiology and biotechnology · 2022Review
- Phage single-gene lysis: Finding the weak spot in the bacterial cell wall.The Journal of biological chemistry · 2019Review
- RNA Phage Biology in a Metagenomic Era.Viruses · 2018Review
- Mutational analysis of the MS2 lysis protein L.Microbiology (Reading, England) · 2017Article
- MS2 Lysis of Escherichia coli Depends on Host Chaperone DnaJ.Journal of bacteriology · 2017Article
- ydfD encodes a novel lytic protein in Escherichia coli.FEMS microbiology letters · 2016Article
- Combining Genes from Multiple Phages for Improved Cell Lysis and DNA Transfer from Escherichia coli to Bacillus subtilis.PloS one · 2016Article
- Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli.Microbiology and molecular biology reviews : MMBR · 1998Review
- From growth to autolysis: the murein hydrolases in Escherichia coli.Archives of microbiology · 1995Review
- Modification of membrane permeability by animal viruses.Advances in virus research · 1995Review
- C9-mediated killing of bacterial cells by transferred C5b-8 complexes: transferred C5b-9 complexes are nonbactericidal.Infection and immunity · 1994Article
- Location and unusual membrane topology of the immunity protein of the Escherichia coli phage T4.Journal of virology · 1993Article
- Bacteriophage lysis: mechanism and regulation.Microbiological reviews · 1992Review
- Subcellular distribution of the soluble lytic transglycosylase in Escherichia coli.Journal of bacteriology · 1991Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was purified by preabsorption to membranes from cells harboring a control plasmid. Protein A-gold was used to label the protein-antibody complexes. Between 42.8% (Lowicryl) and 33.8% (Epon) of the label was found in inner and outer membranes, but 30.3% (Lowicryl) and 32.8% (Epon) was present mostly in clusters in the adhesion sites visible after plasmolysis. The remaining label (26.9 and 33.4%, respectively) appeared to be present in the periplasmic space but may also have been part of membrane junctions not visible because of poor contrast of the specimen. In contrast, a quite different distribution of the L protein was found in cells grown under conditions of penicillin tolerance, i.e., at pH 5, a condition that had previously been shown to protect cells from L-protein-induced lysis. At tolerant conditions, only 21.0% of the L protein was in the adhesion sites; most of the protein (68.2%) was found in inner and outer membranes. It is concluded that lysis of the host, E. coli, was a result of the formation of specific L-protein-mediated membrane adhesion sites.
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