ArticlePLoS neglected tropical diseases2015
Production of Infectious Dengue Virus in Aedes aegypti Is Dependent on the Ubiquitin Proteasome Pathway.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Distinct 5' UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses.Microbiology and immunology · 2026Article
- Direct Detection ofSensors (Basel, Switzerland) · 2025Article
- Ubiquitination of NS1 Confers Differential Adaptation of Zika Virus in Mammalian Hosts and Mosquito Vectors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Integrated One Health strategies in Dengue.One health (Amsterdam, Netherlands) · 2024Review
- Article
- Pathogenesis of Zika Virus Infection.Annual review of pathology · 2023Review
- Influence of Host Blood Meal Source on Gut Microbiota of Wild CaughtMicroorganisms · 2022Article
- Metabolic Processes Are Differentially Regulated During Wild-Type and Attenuated Dengue Virus Infection in Aedes aegypti.The American journal of tropical medicine and hygiene · 2022Article
- Dual-Role Ubiquitination Regulation Shuttling the Entire Life Cycle of the Flaviviridae.Frontiers in microbiology · 2022Review
- Establishing functional lentiviral vector production in a stirred bioreactor for CAR-T cell therapy.Bioengineered · 2021Article
- How Viruses Use the VCP/p97 ATPase Molecular Machine.Viruses · 2021Review
- Uncovering the Worldwide Diversity and Evolution of the Virome of the MosquitoesMicroorganisms · 2021Article
- Comprehensive Quantitative Proteome Analysis ofFrontiers in physiology · 2021Article
- Production, Transmission, Pathogenesis, and Control of Dengue Virus: A Literature-Based Undivided Perspective.BioMed research international · 2021Review
- Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure.PLoS neglected tropical diseases · 2020Article
- TRIM Proteins in Host Defense and Viral Pathogenesis.Current clinical microbiology reports · 2020Review
- Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti.PLoS pathogens · 2019Article
- Antibody-Dependent Dengue Virus Entry Modulates Cell Intrinsic Responses for Enhanced Infection.mSphere · 2019Article
- The Mosquito Immune System and the Life of Dengue Virus: What We Know and Do Not Know.Pathogens (Basel, Switzerland) · 2019Review
- Quantitative Comparative Proteomics Reveal Biomarkers for Dengue Disease Severity.Frontiers in microbiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue virus (DENV) relies on host factors to complete its life cycle in its mosquito host for subsequent transmission to humans. DENV first establishes infection in the midgut of Aedes aegypti and spreads to various mosquito organs for lifelong infection. Curiously, studies have shown that infectious DENV titers peak and decrease thereafter in the midgut despite relatively stable viral genome levels. However, the mechanisms that regulate this decoupling of infectious virion production from viral RNA replication have never been determined. We show here that the ubiquitin proteasome pathway (UPP) plays an important role in regulating infectious DENV production. Using RNA interference studies, we show in vivo that knockdown of selected UPP components reduced infectious virus production without altering viral RNA replication in the midgut. Furthermore, this decoupling effect could also be observed after RNAi knockdown in the head/thorax of the mosquito, which otherwise showed direct correlation between infectious DENV titer and viral RNA levels. The dependence on the UPP for successful DENV production is further reinforced by the observed up-regulation of key UPP molecules upon DENV infection that overcome the relatively low expression of these genes after a blood meal. Collectively, our findings indicate an important role for the UPP in regulating DENV production in the mosquito vector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.