ArticlePloS one2015
Variation in Taxonomic Composition of the Fecal Microbiota in an Inbred Mouse Strain across Individuals and Time.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Gut microbiome modulates breast cancer risk in socially isolated mice.Breast cancer research : BCR · 2026Article
- Breaking down barriers: is intestinal mucus degradation byInfection and immunity · 2025Review
- Laboratory mice engrafted with natural gut microbiota possess a wildling-like phenotype.Nature communications · 2025Article
- Influence of Water Delivery Method on the Gut Microbiome in Laboratory Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Effect size of delayed freezing, diurnal variation, and hindgut location on the mouse fecal microbiome.iScience · 2024Article
- Article
- Risk of bias assessment tool for systematic review and meta-analysis of the gut microbiome.Gut microbiome (Cambridge, England) · 2023Article
- Resilience or susceptibility to traumatic stress: Potential influence of the microbiome.Neurobiology of stress · 2022Article
- Mapping the Influence of the Gut Microbiota on Small Molecules across the Microbiome Gut Brain Axis.Journal of the American Society for Mass Spectrometry · 2022Article
- Article
- Cage Environment Regulates Gut Microbiota Independent of Toll-Like Receptors.Infection and immunity · 2021Article
- Shared and distinctive features of the gut microbiome of C57BL/6 mice from different vendors and production sites, and in response to a new vivarium.Lab animal · 2021Article
- Longitudinal Changes in Diet Cause Repeatable and Largely Reversible Shifts in Gut Microbial Communities of Laboratory Mice and Are Observed across Segments of the Entire Intestinal Tract.International journal of molecular sciences · 2021Article
- Cage and maternal effects on the bacterial communities of the murine gut.Scientific reports · 2021Article
- Article
- Temporospatial shifts within commercial laboratory mouse gut microbiota impact experimental reproducibility.BMC biology · 2020Article
- Toll-like receptor-2 and -4 responses regulate neutrophil infiltration into the junctional epithelium and significantly contribute to the composition of the oral microbiota.Journal of periodontology · 2019Article
- Murine Genetic Background Has a Stronger Impact on the Composition of the Gut Microbiota than Maternal Inoculation or Exposure to Unlike Exogenous Microbiota.Applied and environmental microbiology · 2019Article
- Latent-period stool proteomic assay of multiple sclerosis model indicates protective capacity of host-expressed protease inhibitors.Scientific reports · 2019Article
- Colitis-induced colorectal cancer and intestinal epithelial estrogen receptor beta impact gut microbiota diversity.International journal of cancer · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Genetics, diet, and other environmental exposures are thought to be major factors in the development and composition of the intestinal microbiota of animals. However, the relative contributions of these factors in adult animals, as well as variation with time in a variety of important settings, are still not fully understood. We studied a population of inbred, female mice fed the same diet and housed under the same conditions. We collected fecal samples from 46 individual mice over two weeks, sampling four of these mice for periods as long as 236 days for a total of 190 samples, and determined the phylogenetic composition of their microbial communities after analyzing 1,849,990 high-quality pyrosequencing reads of the 16S rRNA gene V3 region. Even under these controlled conditions, we found significant inter-individual variation in community composition, as well as variation within an individual over time, including increases in alpha diversity during the first 2 months of co-habitation. Some variation was explained by mouse membership in different cage and vendor shipment groups. The differences among individual mice from the same shipment group and cage were still significant. Overall, we found that 23% of the variation in intestinal microbiota composition was explained by changes within the fecal microbiota of a mouse over time, 12% was explained by persistent differences among individual mice, 14% by cage, and 18% by shipment group. Our findings suggest that the microbiota of controlled populations of inbred laboratory animals may not be as uniform as previously thought, that animal rearing and handling may account for some variation, and that as yet unidentified factors may explain additional components of variation in the composition of the microbiota within populations and individuals over time. These findings have implications for the design and interpretation of experiments involving laboratory animals.
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Registered trials
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