ArticleThe Journal of biological chemistry2016
In Vitro Characterization of the Type I Toxin-Antitoxin System bsrE/SR5 from Bacillus subtilis.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Design of orthogonal and portable RNA devices for post-transcriptional regulation by reverse engineering type I toxin-antitoxin systems.Nucleic acids research · 2025Article
- A high-resolution view of RNA endonuclease cleavage in Bacillus subtilis.Nucleic acids research · 2025Article
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- Regulatory RNAs inSynthetic and systems biotechnology · 2024Review
- In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- T1TAdb: the database of type I toxin-antitoxin systems.RNA (New York, N.Y.) · 2021Article
- Review
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- Toxin⁻Antitoxin Systems inToxins · 2019Review
- Type I Toxin-Antitoxin Systems in Clostridia.Toxins · 2019Review
- Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus.Nucleic acids research · 2019Article
- Type I Toxin-Antitoxin Systems: Regulating Toxin Expression via Shine-Dalgarno Sequence Sequestration and Small RNA Binding.Microbiology spectrum · 2018Review
- Discovery of new type I toxin-antitoxin systems adjacent to CRISPR arrays in Clostridium difficile.Nucleic acids research · 2018Article
- Article
- Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression.Microbiology and molecular biology reviews : MMBR · 2016Review
- A multistress responsive type I toxin-antitoxin system: bsrE/SR5 from the B. subtilis chromosome.RNA biology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BsrE/SR5 is a new type I toxin/antitoxin system located on the prophage-like region P6 of the Bacillus subtilis chromosome. The bsrE gene encoding a 30-amino acid hydrophobic toxin and the antitoxin gene sr5 overlap at their 3' ends by 112 bp. Overexpression of bsrE causes cell lysis on agar plates. Here, we present a detailed in vitro analysis of bsrE/SR5. The secondary structures of SR5, bsrE mRNA, and the SR5/bsrE RNA complex were determined. Apparent binding rate constants (kapp) of wild-type and mutated SR5 species with wild-type bsrE mRNA were calculated, and SR5 regions required for efficient inhibition of bsrE mRNA narrowed down. In vivo studies confirmed the in vitro data but indicated that a so far unknown RNA binding protein might exist in B. subtilis that can promote antitoxin/toxin RNA interaction. Using time course experiments, the binding pathway of SR5 and bsrE RNA was elucidated. A comparison with the previously well characterized type I TA system from the B. subtilis chromosome, bsrG/SR4, reveals similarities but also significant differences.
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