ArticleG3 (Bethesda, Md.)2015
Genome-Wide Estimates of Mutation Rates and Spectrum in Schizosaccharomyces pombe Indicate CpG Sites are Highly Mutagenic Despite the Absence of DNA Methylation.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 61 citations in OpenAlex.
- Parallel but distinct adaptive routes in the budding and fission yeasts after 10,000 generations of experimental evolution.Nature ecology & evolution · 2026Article
- Elevated rates and biased spectra of mutations in anaerobically cultured lactic acid bacteria.mBio · 2025Article
- Contrasting mutation patterns in haploid and diploid cells from two yeast species.bioRxiv : the preprint server for biology · 2025Article
- The negligible mutagenic effects of norfloxacin on the genome of the fission yeastMicrobiology spectrum · 2025Article
- What can we infer about mutation calling by using time-series mutation accumulation data and a Bayesian Mutation Finder?Ecology and evolution · 2024Article
- Evolution of a minimal cell.Nature · 2023Article
- Very Low Rates of Spontaneous Gene Deletions and Gene Duplications in Dictyostelium discoideum.Journal of molecular evolution · 2023Article
- Genome-wide analysis of heat stress-stimulated transposon mobility in the human fungal pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- "Bet hedging" against climate change in developing and adult animals: roles for stochastic gene expression, phenotypic plasticity, epigenetic inheritance and adaptation.Frontiers in physiology · 2023Review
- Intrinsic base substitution patterns in diverse species reveal links to cancer and metabolism.Genetics · 2022Article
- Recent insights into the evolution of mutation rates in yeast.Current opinion in genetics & development · 2022Review
- Staggered intercalation of DNA duplexes with base-pair modulation by two distinct drug molecules induces asymmetric backbone twisting and structure polymorphism.Nucleic acids research · 2022Article
- Species-Specific Paternal Age Effects and Sperm Methylation Levels of Developmentally Important Genes.Cells · 2022Article
- Epigenetic modifications affect the rate of spontaneous mutations in a pathogenic fungus.Nature communications · 2021Article
- Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.PLoS genetics · 2021Article
- Reference genome assembly for Australian Ascochyta lentis isolate Al4.G3 (Bethesda, Md.) · 2021Article
- Variable Spontaneous Mutation and Loss of Heterozygosity among Heterozygous Genomes in Yeast.Molecular biology and evolution · 2020Article
- Low Base-Substitution Mutation Rate but High Rate of Slippage Mutations in the Sequence Repeat-Rich Genome ofG3 (Bethesda, Md.) · 2020Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We accumulated mutations for 1952 generations in 79 initially identical, haploid lines of the fission yeast Schizosaccharomyces pombe, and then performed whole-genome sequencing to determine the mutation rates and spectrum. We captured 696 spontaneous mutations across the 79 mutation accumulation (MA) lines. We compared the mutation spectrum and rate to a recently published equivalent experiment on the same species, and to another model ascomycetous yeast, the budding yeast Saccharomyces cerevisiae. While the two species are approximately 600 million years diverged from each other, they share similar life histories, genome size and genomic G/C content. We found that Sc. pombe and S. cerevisiae have similar mutation rates, but Sc. pombe exhibits a stronger insertion bias. Intriguingly, we observed an increased mutation rate at cytosine nucleotides, specifically CpG nucleotides, which is also seen in S. cerevisiae. However, the absence of methylation in Sc. pombe and the pattern of mutation at these sites, primarily C → A as opposed to C → T, strongly suggest that the increased mutation rate is not caused by deamination of methylated cytosines. This result implies that the high mutability of CpG dinucleotides in other species may be caused in part by a methylation-independent mechanism. Many of our findings mirror those seen in the recent study, despite the use of different passaging conditions, indicating that MA is a reliable method for estimating mutation rates and spectra.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.