Evidence map›Paper›PMID 26564796›Full record

ArticleMolecular biology of the cell2016

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Shan Xu, Edward Cherok, Shweta Das, Sunan Li, Brian A Roelofs, Shealinna X Ge, Brian M Polster, Liron Boyman, W Jonathan Lederer, Chunxin Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
97citing papers in PubMed, 1 pooled it
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

97 citing papers in PubMed, 1 synthesis or guideline pooled it, 141 citations in OpenAlex.

  1. Pooled it
  2. Review
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  7. Review
  8. Article
  9. MARCH E3 Ligases: Understudied Regulators of Pulmonary Immune Function.Journal of respiratory biology and translational medicine · 2026
    Article
  10. Subcellular Stress Markers in Epithelial Ovarian Cancer.International journal of molecular sciences · 2025
    Review
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  14. Article
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37 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Shan XuCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201.
Edward CherokCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201.
Shweta DasCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201.
Sunan LiCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201.
Brian A RoelofsCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Anesthesiology and the Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, Baltimore, MD 21201.
Shealinna X GeDepartment of Anesthesiology and the Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, Baltimore, MD 21201.
Brian M PolsterDepartment of Anesthesiology and the Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, Baltimore, MD 21201.
Liron BoymanCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201.
W Jonathan LedererCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201.
Chunxin WangBiochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Mariusz KarbowskiCenter for Biomedical Engineering and Technology, University of Maryland School of Medicine, Baltimore, MD 21201 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201 mkarbowski@umaryland.edu.
University of Maryland, Baltimore · USNational Institutes of Health · US

Funding

Calcium Entrained ArrhythmiasR01HL105239 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI JAFRI, MOHSIN SALEET, LEDERER, WILLIAM JONATHAN · 2011 to 2015
$4.9M
Multiscale spatiotemporal modeling of cardiac mitochondriaU01HL116321 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI JAFRI, MOHSIN SALEET, LEDERER, WILLIAM JONATHAN · 2014 to 2018
$3.5M
Novel Mechanisms of Microglial Neurotoxicity at Physiological OxygenR01NS085165 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI POLSTER, BRIAN M · 2013 to 2017
$1.7M
The role of mitochondria-associated RING finger proteins in mitochondrial qualityR01GM083131 · NIGMS · UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE · PI KARBOWSKI, MARIUSZ · 2008 to 2012
$1.3M
Control of mitochondrial proteostasis by AAA-ATPase p97R01GM102177 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI KARBOWSKI, MARIUSZ · 2012 to 2015
$1.2M
Intramural NIH HHSNHLBI NIH HHS R01 HL105239NHLBI NIH HHS U01 HL116321NIGMS NIH HHS R01 GM083131NIGMS NIH HHS R01 GM102177NINDS NIH HHS R01 NS085165
6 · The paper itself

Abstract

Ubiquitin- and proteasome-dependent outer mitochondrial membrane (OMM)-associated degradation (OMMAD) is critical for mitochondrial and cellular homeostasis. However, the scope and molecular mechanisms of the OMMAD pathways are still not well understood. We report that the OMM-associated E3 ubiquitin ligase MARCH5 controls dynamin-related protein 1 (Drp1)-dependent mitochondrial fission and cell sensitivity to stress-induced apoptosis. MARCH5 knockout selectively inhibited ubiquitination and proteasomal degradation of MiD49, a mitochondrial receptor of Drp1, and consequently led to mitochondrial fragmentation. Mitochondrial fragmentation in MARCH5(-/-) cells was not associated with inhibition of mitochondrial fusion or bioenergetic defects, supporting the possibility that MARCH5 is a negative regulator of mitochondrial fission. Both MARCH5 re-expression and MiD49 knockout in MARCH5(-/-) cells reversed mitochondrial fragmentation and reduced sensitivity to stress-induced apoptosis. These findings and data showing MARCH5-dependent degradation of MiD49 upon stress support the possibility that MARCH5 regulation of MiD49 is a novel mechanism controlling mitochondrial fission and, consequently, the cellular response to stress.

Indexed as

ApoptosisDynaminsGTP PhosphohydrolasesHCT116 CellsHeLa CellsHomeostasisHumansMembrane ProteinsMicrotubule-Associated ProteinsMitochondriaMitochondrial DynamicsMitochondrial MembranesMitochondrial ProteinsPeptide Elongation FactorsStress, PhysiologicalUbiquitinationDNM1L protein, humanDynaminsGTP PhosphohydrolasesMARCHF5 protein, humanMembrane ProteinsMicrotubule-Associated ProteinsMIEF2 protein, humanMitochondrial ProteinsPeptide Elongation FactorsUbiquitin-Protein Ligases

Identifiers

PMID26564796
PMCPMC4713136
OpenAlexW2221433949

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.