ArticleScientific reports2015
X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- X-ray histology on rapidly fixed fresh tumor tissue samples for fast resection margin assessment.Scientific reports · 2026Article
- Quantitative 3D assessment of muscle dystrophy through X-ray phase-contrast tomography.Scientific reports · 2025Article
- X-ray phase contrast reveals soft tissue and shell growth lines in mollusks.Communications biology · 2024Article
- Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants.Frontiers in endocrinology · 2023Article
- Acquisition of a single grid-based phase-contrast X-ray image using instantaneous frequency and noise filtering.Biomedical engineering online · 2022Article
- Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish.Cardiovascular research · 2022Article
- Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve.Journal of medical imaging (Bellingham, Wash.) · 2022Article
- Can laboratory x-ray virtual histology provide intraoperative 3D tumor resection margin assessment?Journal of medical imaging (Bellingham, Wash.) · 2022Article
- A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography.Scientific reports · 2021Article
- X-ray Micro-Computed Tomography: An Emerging Technology to Analyze Vascular Calcification in Animal Models.International journal of molecular sciences · 2020Review
- A phase-retrieval toolbox for X-ray holography and tomography.Journal of synchrotron radiation · 2020Article
- Phase-contrast x-ray tomography of neuronal tissue at laboratory sources with submicron resolution.Journal of medical imaging (Bellingham, Wash.) · 2020Article
- Enhanced contrast in X-ray microtomographic images of the membranous labyrinth using different X-ray sources and scanning modes.Journal of anatomy · 2018Article
- Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography.Scientific reports · 2018Article
- High resolution laboratory grating-based X-ray phase-contrast CT.Scientific reports · 2018Article
- Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography.Journal of visualized experiments : JoVE · 2018Article
- 3D multiscale imaging of human vocal folds using synchrotron X-ray microtomography in phase retrieval mode.Scientific reports · 2018Article
- Cellular-resolution 3D virtual histology of human coronary arteries using x-ray phase tomography.Scientific reports · 2018Article
- A Gaussian extension for Diffraction Enhanced Imaging.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging of muscular structure with cellular or subcellular detail in whole-body animal models is of key importance for understanding muscular disease and assessing interventions. Classical histological methods for high-resolution imaging methods require excision, fixation and staining. Here we show that the three-dimensional muscular structure of unstained whole zebrafish can be imaged with sub-5 μm detail with X-ray phase-contrast tomography. Our method relies on a laboratory propagation-based phase-contrast system tailored for detection of low-contrast 4-6 μm subcellular myofibrils. The method is demonstrated on 20 days post fertilization zebrafish larvae and comparative histology confirms that we resolve individual myofibrils in the whole-body animal. X-ray imaging of healthy zebrafish show the expected structured muscle pattern while specimen with a dystrophin deficiency (sapje) displays an unstructured pattern, typical of Duchenne muscular dystrophy. The method opens up for whole-body imaging with sub-cellular detail also of other types of soft tissue and in different animal models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.