Evidence map›Paper›PMID 26564126›Full record

ArticleSleep2016

RGS Proteins and Gαi2 Modulate Sleep, Wakefulness, and Disruption of Sleep/ Wake States after Isoflurane and Sevoflurane Anesthesia.

Hao Zhang, Heather Wheat, Peter Wang, Sha Jiang, Helen A Baghdoyan, Richard R Neubig, X Y Shi, Ralph Lydic

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sleep, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Trial
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Hao ZhangDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
Heather WheatDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
Peter WangDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
Sha JiangDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
Helen A BaghdoyanDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
Richard R NeubigDepartment of Pharmacology and Toxicology, Michigan State University, Lansing, MI.
X Y ShiDepartment of Anesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Ralph LydicDepartment of Anesthesiology, University of Michigan, Ann Arbor, MI.
University of Michigan–Ann Arbor · USMichigan State University · USSecond Military Medical University · CNUniversity of Tennessee at Knoxville · US

Funding

CHOLINERGIC MECHANISMS OF REM SLEEP GENERATIONR37MH045361 · NIMH · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI BAGHDOYAN, HELEN A · 2004 to 2013
$3.4M
CHOLINERGIC PHENOTYPE IN MURINE MODELS OF SLEEPR01HL065272 · NHLBI · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI LYDIC, RALPH · 1999 to 2013
$3.2M
CHOLINERGIC MECHANISMS OF REM SLEEP GENERATIONR01MH045361 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BAGHDOYAN, HELEN A · 1994 to 2003
$807k
CHOLINERGIC MECHANISMS OF REM SLEEP GENERATIONR29MH045361 · NIMH · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI BAGHDOYAN, HELEN A · 1989 to 1993
–
NHLBI NIH HHS HL65272NIGMS NIH HHS GM039561NIMH NIH HHS MH45361
6 · The paper itself

Abstract

STUDY

objectivesThis study tested the hypothesis that Regulators of G protein Signaling (RGS) proteins contribute to the regulation of wakefulness, non-rapid eye movement (NREM) sleep, and rapid eye movement (REM) sleep, and to sleep disruption caused by volatile anesthetics.

methodsThe three groups used in this study included wild-type (WT; n = 7) mice and knock-in mice that were heterozygous (+/GS; n = 7) or homozygous (GS/GS; n = 7) for an RGS-insensitive allele that causes prolonged Gαi2 signaling. Mice were implanted with electrodes for recording sleep and conditioned for 1 week or more to sleep in the laboratory. Using within and between groups designs, 24-h recordings of wakefulness, NREM sleep, and REM sleep were compared across three interventions: (1) baseline (control) and after 3 h of being anesthetized with (2) isoflurane or (3) sevoflurane.

resultsBaseline recordings during the light phase revealed that relative to WT mice, homozygous RGS-insensitive (GS/GS) mice exhibit significantly increased wakefulness and decreased NREM and REM sleep. During the dark phase, these state-specific differences remained significant but reversed direction of change. After cessation of isoflurane and sevoflurane anesthesia there was a long-lasting and significant disruption of sleep and wakefulness. The durations of average episodes of wakefulness, NREM sleep, and REM sleep were significantly altered as a function of genotype and isoflurane and sevoflurane anesthesia.

conclusionsRGS proteins and Gαi2 play a significant role in regulating states of wakefulness, NREM sleep, and REM sleep. Genotype-specific differences demonstrate that RGS proteins modulate sleep disruption caused by isoflurane and sevoflurane anesthesia. The results also support the conclusion that isoflurane and sevoflurane anesthesia do not satisfy the homeostatic drive for sleep.

Indexed as

AnesthesiaAnimalsElectrodes, ImplantedElectroencephalographyGTP-Binding Protein alpha Subunit, Gi2HomeostasisIsofluraneMaleMethyl EthersMiceRGS ProteinsSevofluraneSleepSleep, REMWakefulnessGTP-Binding Protein alpha Subunit, Gi2IsofluraneMethyl EthersRGS ProteinsSevofluraneG proteinsmetabotropic receptorsshared circuits hypothesissignal transduction

Identifiers

PMID26564126
PMCPMC4712393
OpenAlexW2335928443

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.