ArticleScientific reports2015
CalQuo: automated, simultaneous single-cell and population-level quantification of global intracellular Ca2+ responses.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- B Cells are Activated via a Nanoporous Interface that Stabilizes Microvilli and Engages Mechanosensitive Ion Channels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Development of a Probability-Based In Vitro Eye Irritation Screening Platform.Bioengineering (Basel, Switzerland) · 2024Article
- Antibody agonists trigger immune receptor signaling through local exclusion of receptor-type protein tyrosine phosphatases.Immunity · 2024Article
- Distinct actin cytoskeleton behaviour in primary and immortalised T-cells.Journal of cell science · 2019Article
- CD45 exclusion- and cross-linking-based receptor signaling together broaden FcεRI reactivity.Science signaling · 2018Article
- Single-Molecule Light-Sheet Imaging of Suspended T Cells.Biophysical journal · 2018Article
- CalQuoScientific reports · 2017Article
- Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation.Science advances · 2017Article
- Dissecting the actin cortex density and membrane-cortex distance in living cells by super-resolution microscopy.Journal of physics D: Applied physics · 2017Article
- Systematic Characterization of Dynamic Parameters of Intracellular Calcium Signals.Frontiers in physiology · 2016Article
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Authors and funding
8 authors.
Funding
Abstract
Detecting intracellular calcium signaling with fluorescent calcium indicator dyes is often coupled with microscopy techniques to follow the activation state of non-excitable cells, including lymphocytes. However, the analysis of global intracellular calcium responses both at the single-cell level and in large ensembles simultaneously has yet to be automated. Here, we present a new software package, CalQuo (Calcium Quantification), which allows the automated analysis and simultaneous monitoring of global fluorescent calcium reporter-based signaling responses in up to 1000 single cells per experiment, at temporal resolutions of sub-seconds to seconds. CalQuo quantifies the number and fraction of responding cells, the temporal dependence of calcium signaling and provides global and individual calcium-reporter fluorescence intensity profiles. We demonstrate the utility of the new method by comparing the calcium-based signaling responses of genetically manipulated human lymphocytic cell lines.
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