Evidence map›Paper›PMID 26563450›Full record

ArticleJournal of molecular neuroscience : MN2016

TRAM1 Promotes Microglia M1 Polarization.

Hanxiang Wang, Chun Liu, Ming Han, Chun Cheng, Dongmei Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular neuroscience : MN, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hanxiang WangJiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, China.
Chun LiuJiangsu Province Laboratory Animal Center, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, China.
Ming HanJiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, China.
Chun ChengJiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, China. 1402339591@qq.com.
Dongmei ZhangJiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, Jiangsu Province, 226001, China. zdm@ntu.edu.cn.
Nantong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microglia, the major immune cells of the central nervous system (CNS), can be driven to adopt M1 and M2 phenotypes. Recently, the distinct functions of M1 and M2 microglia have been intensively studied. M1-activation microglia are pro-inflammatory and may contribute to the development of several CSN disorders, while M2-activation microglia are anti-inflammatory and may promote tissue reconstruction. TRAM1 is a protein involved in translocation of nascent polypeptides and functions as a sorting adaptor of TLR4. Here, we found that TRAM1 plays an important role in microglia M1 polarization. Our results showed that the expression of TRAM1 is highly induced in LPS/interferon (IFN)-γ-stimulated BV2 cells and primary microglia cells. Flag-TRAM1 transfection, but not Flag-GFP used as a control, significantly enhanced M1 polarization by strongly increasing expression of M1 makers, such as IL-6, IL-1β, iNOS, and so on. Silence of TRAM1 effectively inhibited LPS/IFN-γ-induced expression of M1-related genes in BV2 cells. In addition, TRAM1 was found to cooperate with TLR4 to induce an M1 genetic program in Flag-TRAM1-transfected and LPS/IFN-γ-induced BV2 cells. TRAM1 is essential for LPS/IFN-γ induced expressions of adapter molecule (IRAK1, phosphorylation of TBK1, and IRF3) of TLR4. TRAM1 is also essential for phosphorylation of IκB and P65 and for P65-NF-kB translocation to nucleus. Overall, our findings showed that TRAM1 could promote microglia M1 polarization.

Indexed as

Cell DifferentiationAnimalsCell LineCells, CulturedInterferon-gammaInterleukin-1 Receptor-Associated KinasesInterleukinsMembrane GlycoproteinsMembrane Transport ProteinsMiceMice, Inbred C57BLMicrogliaNF-kappa BNitric Oxide Synthase Type IIToll-Like Receptor 4Interferon-gammaInterleukin-1 Receptor-Associated KinasesInterleukinsIrak1 protein, mouseMembrane GlycoproteinsMembrane Transport ProteinsNF-kappa BNitric Oxide Synthase Type IITlr4 protein, mouseToll-Like Receptor 4TRAM1 protein, mouseINOSLPS/IFN-γMicroglia M1 polarizationTBKTRAM1

Identifiers

PMID26563450
OpenAlexW2242800549

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.