ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Development of an ex vivo breast cancer lung colonization model utilizing a decellularized lung matrix.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Applications of lung cancer organoids in precision medicine: from bench to bedside.Cell communication and signaling : CCS · 2023Pooled it
- Mutant p53 regulates cancer cell invasion in complex three-dimensional environments through mevalonate pathway-dependent Rho/ROCK signaling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Acellular Extracellular Matrix Scaffolds in Regenerative Medicine: Advances in Decellularization and Clinical Applications.Journal of functional biomaterials · 2025Review
- Exploring cell dynamics and tumor microenvironments: a comprehensive review of decellularized extracellular matrix (dECM) scaffolds in breast and prostate cancer research.Research in pharmaceutical sciences · 2025Review
- Hsp47 drives obesity-associated breast cancer progression by enhancing asporin deposition in adipose tissue.Breast cancer research : BCR · 2025Article
- Revealing the structural microenvironment of high metastatic risk uveal melanomas following decellularisation.Scientific reports · 2024Article
- State of the Art Modelling of the Breast Cancer Metastatic Microenvironment: Where Are We?Journal of mammary gland biology and neoplasia · 2024Review
- Three-Dimensional Cultivation a Valuable Tool for Modelling Canine Mammary Gland Tumour Behaviour In Vitro.Cells · 2024Review
- Review
- Article
- Novel Decellularization Method for Tissue Slices.Frontiers in bioengineering and biotechnology · 2022Article
- Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications.Biomedicines · 2021Article
- Three-Dimensional Vascularized Lung Cancer-on-a-Chip with Lung Extracellular Matrix Hydrogels for In Vitro Screening.Cancers · 2021Article
- Image-Based Method to Quantify Decellularization of Tissue Sections.International journal of molecular sciences · 2021Article
- Phenotypic Heterogeneity and Metastasis of Breast Cancer Cells.Cancer research · 2021Article
- Analysis of structural components of decellularized scaffolds in renal fibrosis.Bioactive materials · 2021Article
- Tumor organoid models in precision medicine and investigating cancer-stromal interactions.Pharmacology & therapeutics · 2021Review
- Patient-Derived Scaffolds of Colorectal Cancer Metastases as an Organotypic 3D Model of the Liver Metastatic Microenvironment.Cancers · 2020Article
- Tracking Extracellular Matrix Remodeling in Lungs Induced by Breast Cancer Metastasis. Fourier Transform Infrared Spectroscopic Studies.Molecules (Basel, Switzerland) · 2020Article
- Organotypic Modeling of the Tumor Landscape.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The metastatic spread of cancer cells to distant sites represents the major cause of cancer-related deaths in breast cancer patients, and lungs are one of the most common sites for metastatic colonization. Developing a physiologically relevant tissue culture model to mimic lung colonization of breast cancer is crucial for the investigation of the biology of cancer metastasis and evaluation of drug treatment efficacy. Here, we describe an ex vivo lung colonization assay for breast cancer using the native three-dimensional (3D) lung extracellular matrix. The native matrix was isolated from murine lungs using a decellularization technique, and the preservation of extracellular matrix (ECM) composition, integrity and mechanical properties was confirmed. We showed that metastatic MDA-MB 231 and 4T1 cells invaded and colonized in the decellularized lung matrix, whereas only a small mass of non-metastatic MCF7 cells survived under the same condition. Furthermore, knockdown of ZEB1, an epithelial-mesenchymal transition (EMT) inducer, significantly reduced invasion and colonization of MDA-MB 231 cells in the decellularized lung, suggesting an important role of EMT in breast cancer metastasis. We conclude that the decellularized lung retains the biophysical and biochemical properties of the lung ECM and provides a powerful tool to investigate the lung colonization of breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.