ArticlePlanta2016
Overexpression of AtPCS1 in tobacco increases arsenic and arsenic plus cadmium accumulation and detoxification.
Article in Planta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 92 citations in OpenAlex.
- Plant-Microbe-Assisted Approaches for Remediating Heavy Metals and Organic Pollutant Contamination in Electronic Waste Dump Sites.ACS omega · 2026Review
- CiPCS regulated by CiMYB4 enhances the tolerance of Chrysanthemum indicum to cadmium stress.Plant cell reports · 2025Article
- Phytochelatins and Cadmium Mitigation: Harnessing Genetic Avenues for Plant Functional Manipulation.International journal of molecular sciences · 2025Review
- Characterization of aFrontiers in plant science · 2024Article
- Synergistic Reduction of Arsenic Uptake and Alleviation of Leaf Arsenic Toxicity in Maize (Journal of fungi (Basel, Switzerland) · 2023Article
- The Effect of Cadmium on Plants in Terms of the Response of Gene Expression Level and Activity.Plants (Basel, Switzerland) · 2023Review
- Tobacco as an efficient metal accumulator.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Insight into the biochemical and physiological mechanisms of nanoparticles-induced arsenic tolerance in bamboo.Frontiers in plant science · 2023Article
- Shoot-root signal circuit: Phytoremediation of heavy metal contaminated soil.Frontiers in plant science · 2023Review
- Sulfur reduces the root-to-shoot translocation of arsenic and cadmium by regulating their vacuolar sequestration in wheat (Frontiers in plant science · 2022Article
- Safe, efficient, and economically beneficial remediation of arsenic-contaminated soil: possible strategies for increasing arsenic tolerance and accumulation in non-edible economically important native plants.Environmental science and pollution research international · 2021Review
- Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants.Environmental science and pollution research international · 2021Review
- Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata.BMC plant biology · 2020Article
- Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular CdBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2019Article
- Recent Trends in Enhancing the Resistance of Cultivated Plants to Heavy Metal Stress by Transgenesis and Transcriptional Programming.Molecular biotechnology · 2019Review
- Expression of the Tobacco Non-symbiotic Class 1 Hemoglobin Gene Hb1 Reduces Cadmium Levels by Modulating Cd Transporter Expression Through Decreasing Nitric Oxide and ROS Level inFrontiers in plant science · 2019Article
- Genetic-based dissection of arsenic accumulation in maize using a genome-wide association analysis method.Plant biotechnology journal · 2018Article
- Overexpression of a FunctionalFrontiers in plant science · 2018Article
- Genome-Wide Association Study of Cadmium Accumulation at the Seedling Stage in Rapeseed (Frontiers in plant science · 2018Article
- InFrontiers in plant science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThe heterologous expression of AtPCS1 in tobacco plants exposed to arsenic plus cadmium enhances phytochelatin levels, root As/Cd accumulation and pollutants detoxification, but does not prevent root cyto-histological damages. High phytochelatin (PC) levels may be involved in accumulation and detoxification of both cadmium (Cd) and arsenic (As) in numerous plants. Although polluted environments are frequently characterized by As and Cd coexistence, how increased PC levels affect the adaptation of the entire plant and the response of its cells/tissues to a combined contamination by As and Cd needs investigation. Consequently, we analyzed tobacco seedlings overexpressing Arabidopsis phytochelatin synthase1 gene (AtPCS1) exposed to As and/or Cd, to evaluate the levels of PCs and As/Cd, the cyto-histological modifications of the roots and the Cd/As leaf extrusion ability. When exposed to As and/or Cd the plants overexpressing AtPCS1 showed higher PC levels, As plus Cd root accumulation, and detoxification ability than the non-overexpressing plants, but a blocked Cd-extrusion from the leaf trichomes. In all genotypes, As, and Cd in particular, damaged lateral root apices, enhancing cell-vacuolization, causing thinning and stretching of endodermis initial cells. Alterations also occurred in the primary structure region of the lateral roots, i.e., cell wall lignification in the external cortex, cell hypertrophy in the inner cortex, crushing of endodermis and stele, and nuclear hypertrophy. Altogether, As and/or Cd caused damage to the lateral roots (and not to the primary one), with such damage not counteracted by AtPCS1 overexpression. The latter, however, positively affected accumulation and detoxification to both pollutants, highlighting that Cd/As accumulation and detoxification due to PCS1 activity do not reduce the cyto-histological damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.