ArticleNature protocols2015
Measuring antigen presentation in mouse brain endothelial cells ex vivo and in vitro.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- The spatiotemporal transcriptional profiling of murine brain during cerebral malaria progression and after artemisinin treatment.Nature communications · 2025Article
- Microvascular Contributions to Alzheimer Disease Pathogenesis: Is Alzheimer Disease Primarily an Endotheliopathy?Biomolecules · 2023Review
- In vitro model of brain endothelial cell barrier reveals alterations induced by Plasmodium blood stage factors.Parasitology research · 2023Article
- Brain endothelial cells exposure to malaria parasites links type I interferon signalling to antigen presentation, immunoproteasome activation, endothelium disruption, and cellular metabolism.Frontiers in immunology · 2023Article
- Brain endothelial STING1 activation byProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Immunomodulation by endothelial cells - partnering up with the immune system?Nature reviews. Immunology · 2022Review
- Amateur antigen-presenting cells in the tumor microenvironment.Molecular carcinogenesis · 2022Review
- Blood-brain barrier-restricted translocation ofeLife · 2021Article
- Unexpected enhancement of FVIII immunogenicity by endothelial expression in lentivirus-transduced and transgenic mice.Blood advances · 2020Article
- Tissue-specific immunopathology during malaria infection.Nature reviews. Immunology · 2018Review
- Occludin regulates glucose uptake and ATP production in pericytes by influencing AMP-activated protein kinase activity.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Article
- Co-infection with Chikungunya virus alters trafficking of pathogenic CD8EMBO molecular medicine · 2018Article
- PDL1 Fusion Protein Protects Against Experimental Cerebral Malaria via Repressing Over-Reactive CD8Frontiers in immunology · 2018Article
- AFrontiers in immunology · 2018Article
- Antigen Presentation by Vascular Cells.Frontiers in immunology · 2017Review
- Hyperinsulinemia alters insulin receptor presentation and internalization in brain microvascular endothelial cells.Diabetes & vascular disease researchArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently demonstrated that brain endothelial cells cross-present parasite antigen during mouse experimental cerebral malaria (ECM). Here we describe a 2-d protocol to detect cross-presentation by isolating the brain microvessels and incubating them with a reporter cell line that expresses lacZ upon detection of the relevant peptide-major histocompatibility complex. After X-gal staining, a typical positive result consists of hundreds of blue spots, compared with fewer than 20 spots from a naive brain. The assay is generalizable to other disease contexts by using reporter cells that express appropriate specific T cell receptors. Also described is the protocol for culturing endothelial cells from brain microvessels isolated from naive mice. After 7-10 d, an in vitro cross-presentation assay can be performed by adding interferon-γ, antigen (e.g., Plasmodium berghei-infected red blood cells) and reporter cells in sequence over 3 d. This is useful for comparing different antigen forms or for probing the effects of various interventions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.