ArticleProceedings of the National Academy of Sciences of the United States of America2015
Superresolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 83 citations in OpenAlex.
- Epigenetic dynamics of human spermatogenesis and their dysregulation in non-obstructive azoospermia.Protein & cell · 2026Article
- Architecture and regulation of nanoscale chromatin domains.Nature communications · 2026Review
- UV curable resin as a rapid and superior sealant for STORM.Biomedical optics express · 2025Article
- Super-resolution microscopy reveals distinct epigenetic states regulated by estrogen receptor activity.bioRxiv : the preprint server for biology · 2025Article
- Super-resolution microscopy reveals distinct epigenetic states regulated by estrogen receptor activity.Research square · 2025Article
- Deciphering meiotic chromatin organization by SYCP3.Nucleic acids research · 2025Article
- Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.Journal of cell science · 2025Article
- Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Review
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- Transcriptionally active chromatin loops contain both 'active' and 'inactive' histone modifications that exhibit exclusivity at the level of nucleosome clusters.Epigenetics & chromatin · 2024Article
- The First Analysis of Synaptonemal Complexes in Jawless Vertebrates: Chromosome Synapsis and Transcription Reactivation at Meiotic Prophase I in the LampreyLife (Basel, Switzerland) · 2023Article
- Super-Resolution Microscopy of the Synaptonemal Complex within the Caenorhabditis elegans Germline.Journal of visualized experiments : JoVE · 2022Article
- Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy.Nature biomedical engineering · 2022Article
- Studying Chromatin Epigenetics with Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Super-resolution microscopy: a brief history and new avenues.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2022Article
- Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment.Nature communications · 2021Article
- Genome-wide variability in recombination activity is associated with meiotic chromatin organization.Genome research · 2021Article
- Parameter-free molecular super-structures quantification in single-molecule localization microscopy.The Journal of cell biology · 2021Article
- Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides.Bio-protocol · 2020Article
- A Role forCells · 2020Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
During meiosis, homologous chromosomes associate to form the synaptonemal complex (SC), a structure essential for fertility. Information about the epigenetic features of chromatin within this structure at the level of superresolution microscopy is largely lacking. We combined single-molecule localization microscopy (SMLM) with quantitative analytical methods to describe the epigenetic landscape of meiotic chromosomes at the pachytene stage in mouse oocytes. DNA is found to be nonrandomly distributed along the length of the SC in condensed clusters. Periodic clusters of repressive chromatin [trimethylation of histone H3 at lysine (Lys) 27 (H3K27me3)] are found at 500-nm intervals along the SC, whereas one of the ends of the SC displays a large and dense cluster of centromeric histone mark [trimethylation of histone H3 at Lys 9 (H3K9me3)]. Chromatin associated with active transcription [trimethylation of histone H3 at Lys 4 (H3K4me3)] is arranged in a radial hair-like loop pattern emerging laterally from the SC. These loops seem to be punctuated with small clusters of H3K4me3 with an average spread larger than their periodicity. Our findings indicate that the nanoscale structure of the pachytene chromosomes is constrained by periodic patterns of chromatin marks, whose function in recombination and higher order genome organization is yet to be elucidated.
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