ArticlePloS one2015
Widespread Environmental Contamination with Mycobacterium tuberculosis Complex Revealed by a Molecular Detection Protocol.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Non-invasive environmental DNA sampling reveals tuberculosis risks at the human - Great Ape Interface in Africa.Emerging microbes & infections · 2026Article
- A microbial safari: finding evidence ofMicrobiology spectrum · 2026Article
- Genomic survey reveals no detectable bacteriophage activity in Mycobacterium bovis across a large population.FEMS microbiology ecology · 2025Article
- Usnic acid impacts energy production and iron metabolism inmSystems · 2025Article
- Massive cerebral tuberculomas, Pott's disease and hypercalcaemia secondary toBMJ case reports · 2024Article
- Population structure and history ofMicrobiology spectrum · 2024Article
- Occurrence of 40 sanitary indicators in French digestates derived from different anaerobic digestion processes and raw organic wastes from agricultural and urban origin.Frontiers in microbiology · 2024Article
- Detection ofFrontiers in microbiology · 2024Article
- Host-, Environment-, or Human-Related Effects Drive Interspecies Interactions in an Animal Tuberculosis Multi-Host Community Depending on the Host and Season.Transboundary and emerging diseases · 2024Article
- Will we ever eradicate animal tuberculosis?Irish veterinary journal · 2023Article
- The impact ofFrontiers in public health · 2022Review
- The Association between Palmer Drought Severity Index Data and Tuberculosis-like Lesions Occurrence in Mediterranean Hunted Wild Boars.Animals : an open access journal from MDPI · 2021Article
- DoesVeterinary medicine international · 2021Review
- Long-Term Determinants of Tuberculosis in the Ungulate Host Community of Doñana National Park.Pathogens (Basel, Switzerland) · 2020Article
- Epidemiology of Tuberculosis in Multi-Host Wildlife Systems: Implications for Black (Frontiers in veterinary science · 2020Review
- Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis.Veterinary research · 2018Article
- The History ofFrontiers in veterinary science · 2018Review
- Efficacy and Safety of BCG Vaccine for Control of Tuberculosis in Domestic Livestock and Wildlife.Frontiers in veterinary science · 2018Review
- Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): bovine tuberculosis.EFSA journal. European Food Safety Authority · 2017Article
- Evaluation of Molecular Methods for the Detection and Quantification of Pathogen-Derived Nucleic Acids in Sediment.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental contamination with Mycobacterium tuberculosis complex (MTC) has been considered crucial for bovine tuberculosis persistence in multi-host-pathogen systems. However, MTC contamination has been difficult to detect due to methodological issues. In an attempt to overcome this limitation we developed an improved protocol for the detection of MTC DNA. MTC DNA concentration was estimated by the Most Probable Number (MPN) method. Making use of this protocol we showed that MTC contamination is widespread in different types of environmental samples from the Iberian Peninsula, which supports indirect transmission as a contributing mechanism for the maintenance of bovine tuberculosis in this multi-host-pathogen system. The proportion of MTC DNA positive samples was higher in the bovine tuberculosis-infected than in presumed negative area (0.32 and 0.18, respectively). Detection varied with the type of environmental sample and was more frequent in sediment from dams and less frequent in water also from dams (0.22 and 0.05, respectively). The proportion of MTC-positive samples was significantly higher in spring (p<0.001), but MTC DNA concentration per sample was higher in autumn and lower in summer. The average MTC DNA concentration in positive samples was 0.82 MPN/g (CI95 0.70-0.98 MPN/g). We were further able to amplify a DNA sequence specific of Mycobacterium bovis/caprae in 4 environmental samples from the bTB-infected area.
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