ArticleNature communications2015
The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Article
- Kinetochore-microtubule error correction for biorientation: lessons from yeast.Biochemical Society transactions · 2024Review
- Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.Science (New York, N.Y.) · 2023Article
- Functional plasticity in chromosome-microtubule coupling on the evolutionary time scale.Life science alliance · 2023Article
- BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation.The EMBO journal · 2023Article
- Improved Analysis of Cross-Linking Mass Spectrometry Data with Kojak 2.0, Advanced by Integration into the Trans-Proteomic Pipeline.Journal of proteome research · 2023Article
- Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain.Structure (London, England : 1993) · 2022Article
- Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.The Journal of cell biology · 2022Article
- Article
- A Crosslinking Mass Spectrometry Protocol for the Structural Analysis of Microtubule-Associated Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Phospho-regulated Bim1/EB1 interactions trigger Dam1c ring assembly at the budding yeast outer kinetochore.The EMBO journal · 2021Article
- Aurora B switches relative strength of kinetochore-microtubule attachment modes for error correction.The Journal of cell biology · 2021Article
- CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.eLife · 2021Article
- Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.Current biology : CB · 2020Article
- Structural view of the yeast Dam1 complex, a ring-shaped molecular coupler for the dynamic microtubule end.Essays in biochemistry · 2020Review
- High-density chemical cross-linking for modeling protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.PLoS genetics · 2019Article
- Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.Current opinion in cell biology · 2019Review
- The Agrobacterium VirD5 protein hyperactivates the mitotic Aurora kinase in host cells.The New phytologist · 2019Article
- Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ.The Journal of cell biology · 2019Article
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Authors and funding
11 authors.
Funding
Abstract
Accurate segregation of chromosomes during cell division is essential. The Dam1 complex binds kinetochores to microtubules and its oligomerization is required to form strong attachments. It is a key target of Aurora B kinase, which destabilizes erroneous attachments allowing subsequent correction. Understanding the roles and regulation of the Dam1 complex requires structural information. Here we apply cross-linking/mass spectrometry and structural modelling to determine the molecular architecture of the Dam1 complex. We find microtubule attachment is accompanied by substantial conformational changes, with direct binding mediated by the carboxy termini of Dam1p and Duo1p. Aurora B phosphorylation of Dam1p C terminus weakens direct interaction with the microtubule. Furthermore, the Dam1p amino terminus forms an interaction interface between Dam1 complexes, which is also disrupted by phosphorylation. Our results demonstrate that Aurora B inhibits both direct interaction with the microtubule and oligomerization of the Dam1 complex to drive error correction during mitosis.
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