ArticleJournal of clinical microbiology2016
Occurrence of Fungal DNA Contamination in PCR Reagents: Approaches to Control and Decontamination.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Fungal meningoencephalitis caused byMedical mycology case reports · 2026Article
- Animal Species and Identity Testing: Developments, Challenges, and Applications to Non-Human Forensics.Genes · 2025Review
- Article
- What every neuropathologist needs to know: Update on neuro infectious disease workups and consultation resources.Journal of neuropathology and experimental neurology · 2025Review
- Validating digital polymerase chain reaction for 16S rRNA gene amplification from low biomass environmental samples.ISME communications · 2025Article
- Diagnosis and management of invasive fungal diseases by next-generation sequencing: are we there yet?Expert review of molecular diagnostics · 2024Review
- Geothermal ecosystems on Mt. Erebus, Antarctica, support diverse and taxonomically novel biota.FEMS microbiology ecology · 2024Article
- Recent Challenges in Diagnosis and Treatment of Invasive Candidiasis in Neonates.Children (Basel, Switzerland) · 2024Review
- Contamination detection and microbiome exploration with GRIMER.GigaScience · 2022Article
- Defining gut mycobiota for wild animals: a need for caution in assigning authentic resident fungal taxa.Animal microbiome · 2021Article
- Article
- Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC/MSGERC Definitions of Invasive Fungal Infection.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- An Overview of Bioinformatics Tools for DNA Meta-Barcoding Analysis of Microbial Communities of Bioaerosols: Digest for Microbiologists.Life (Basel, Switzerland) · 2020Review
- Impact of ITS-Based Sequencing on Antifungal Treatment of Patients with Suspected Invasive Fungal Infections.Journal of fungi (Basel, Switzerland) · 2020Article
- Diagnostic Mycology: Xtreme Challenges.Journal of clinical microbiology · 2020Review
- Is This Zebra Really a Zebra? The Challenge of Diagnosing Rare Fungal Infections in Veterinary Pathology.Veterinary pathology · 2019Article
- From trash to treasure: detecting unexpected contamination in unmapped NGS data.BMC bioinformatics · 2019Article
- The challenge of molecular diagnosis of bloodstream infections.World journal of microbiology & biotechnology · 2019Review
- Plasmid DNA contaminant in molecular reagents.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid amplification techniques permitting sensitive and rapid screening in patients at risk for invasive fungal infections are an important addition to conventional fungal diagnostic methods. However, contamination with fungal DNA may be a serious threat to the validity of fungal amplification-based assays. Besides rigorous handling procedures to avoid false-positive test results from exogenous sources, we have implemented protocols for comprehensive assessment of fungal contamination in all materials involved in the analytical process. Traces of fungal DNA were found in different commercially available PCR reagents, including lyophilized primers, TaqMan probes, and master mix solutions. These contaminants resulted in a considerable rate of false-positive tests in panfungal real-time PCR analysis. To address this problem, we have established a decontamination protocol based on the activity of a double-strand specific DNase. Using this approach, we have significantly reduced the frequency of false-positive test results attributable to contaminated reagents. On the basis of our findings, we strongly recommend routine monitoring of all reagents used in fungal PCR assays for the presence of relevant contaminants. As long as fungal-grade reagents are not readily available, pretreatment methods facilitating elimination of fungal DNA are critical for reducing the risk of false-positive results in highly sensitive molecular fungal detection assays.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.