Evidence map›Paper›PMID 26560537›Full record

ArticleJournal of clinical microbiology2016

Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.

Ji-Hyeon Hwang, Yong-Keol Shin, So-Yeon Park, Jeesoo Kim, Su-Mi Kim, Byounghan Kim, Jong-Hyeon Park, Jong-Soo Lee, Kwang-Nyeong Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ji-Hyeon HwangFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Yong-Keol ShinEnzynomics, Inc., Daejeon, Republic of Korea.
So-Yeon ParkEnzynomics, Inc., Daejeon, Republic of Korea.
Jeesoo KimFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Su-Mi KimFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Byounghan KimFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Jong-Hyeon ParkFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Jong-Soo LeeVeterinary College, Chungnam National University, Daejeon, Republic of Korea.
Kwang-Nyeong LeeFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea leekwn@korea.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.

Indexed as

Equipment ContaminationAnimalsDecontaminationDNA Restriction EnzymesFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionDNA Restriction Enzymes

Identifiers

PMID26560537
PMCPMC4702723

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.