Evidence map›Paper›PMID 26560533›Full record

ArticleJournal of clinical microbiology2016

Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.

Guoqing Zhang, Fangping Cai, Ivette Lorenzana de Rivera, Zhiyong Zhou, Jing Zhang, John Nkengasong, Feng Gao, Chunfu Yang

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Nucleic acid testing and molecular characterization of HIV infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019
    Review
  7. Diagnosis of Human Immunodeficiency Virus Infection.Clinical microbiology reviews · 2019
    Review
  8. Article
  9. Article
  10. Review
  11. HIV-1 drug resistance and resistance testing.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guoqing ZhangInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
Fangping CaiDepartment of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
Ivette Lorenzana de RiveraMicrobiology Department, National Autonomous University of Honduras, Tegucigalpa, Honduras.
Zhiyong ZhouInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
Jing ZhangInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
John NkengasongInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
Feng GaoDepartment of Medicine, Duke University Medical Center, Durham, North Carolina, USA cyang1@cdc.gov fgao@duke.edu.
Chunfu YangInternational Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA cyang1@cdc.gov fgao@duke.edu.

Funding

Role of Virus Recombination in Multiple Drug ResisitanceR01GM065057 · NIGMS · DUKE UNIVERSITY · PI GAO, FENG · 2003 to 2007
$1.7M
NIGMS NIH HHS R01 GM065057
6 · The paper itself

Abstract

A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.

Indexed as

DesiccationDrug Resistance, ViralMutation, MissenseBloodGenotyping TechniquesHIV-1HIV InfectionsHumansMicrobial Sensitivity TestsSpecimen Handling

Identifiers

PMID26560533
PMCPMC4702749

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.