ArticleIEEE transactions on ultrasonics, ferroelectrics, and frequency control2015
Ultrasonic scattering measurements of a live single cell at 86 MHz.
Article in IEEE transactions on ultrasonics, ferroelectrics, and frequency control, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.Scientific reports · 2022Article
- Recent Advancements in Ultrasound Transducer: From Material Strategies to Biomedical Applications.BME frontiers · 2022Review
- Spectrum analysis for assessing red blood cell aggregation using high-frequency ultrasound array transducer.Biomedical engineering letters · 2017Article
- Label-free analysis of the characteristics of a single cell trapped by acoustic tweezers.Scientific reports · 2017Article
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5 authors.
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Abstract
Cell separation and sorting techniques have been employed biomedical applications such as cancer diagnosis and cell gene expression analysis. The capability to accurately measure ultrasonic scattering properties from cells is crucial in making an ultrasonic cell sorter a reality if ultrasound scattering is to be used as the sensing mechanism as well. To assess the performance of sensing and identifying live single cells with high-frequency ultrasound, an 86-MHz lithium niobate press-focused single-element acoustic transducer was used in a high-frequency ultrasound scattering measurement system that was custom designed and developed for minimizing noise and allowing better mobility. Peak-to-peak echo amplitude, integrated backscatter (IB) coefficient, spectral parameters including spectral slope and intercept, and midband fit from spectral analysis of the backscattered echoes were measured and calculated from a live single cell of two different types on an agar surface: leukemia cells (K562 cells) and red blood cells (RBCs). The amplitudes of echo signals from K562 cells and RBCs were 48.25 ± 11.98 mV(pp) and 56.97 ± 7.53 mV(pp), respectively. The IB coefficient was -89.39 ± 2.44 dB for K562 cells and -89.00 ± 1.19 dB for RBCs. The spectral slope and intercept were 0.30 ± 0.19 dB/MHz and -56.07 ± 17.17 dB, respectively, for K562 cells and 0.78 ± 0.092 dB/MHz and -98.18 ± 8.80 dB, respectively, for RBCs. Midband fits of K562 cells and RBCs were -31.02 ± 3.04 dB and -33.51 ± 1.55 dB, respectively. Acoustic cellular discrimination via these parameters was tested by Student's t-test. Their values, except for the IB value, showed statistically significant difference (p < 0.001). This paper reports for the first time that ultrasonic scattering measurements can be made on a live single cell with a highly focused high-frequency ultrasound microbeam at 86 MHz. These results also suggest the feasibility of ultrasonic scattering as a sensing mechanism in the development of ultrasonic cell sorters.
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