ArticlePlant physiology2016
A Structural Study of CESA1 Catalytic Domain of Arabidopsis Cellulose Synthesis Complex: Evidence for CESA Trimers.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 116 citations in OpenAlex.
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- Uniform elementary fibrils in diverse plant cell walls.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Supramolecular DNA-based catalysis in organic solvents.iScience · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- Discovery and Characterization of Fluopipamine, a Putative Cellulose Synthase 1 Antagonist withinJournal of agricultural and food chemistry · 2024Article
- Tissue-specific directionality of cellulose synthase complex movement inferred from cellulose microfibril polarity in secondary cell walls of Arabidopsis.Scientific reports · 2023Article
- Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.The Journal of cell biology · 2023Article
- Essential amino acids in the Plant-Conserved and Class-Specific Regions of cellulose synthases.Plant physiology · 2023Article
- Mutation in the Endo-β-1,4-glucanase (KORRIGAN) Is Responsible for Thick Leaf Phenotype in Sorghum.Plants (Basel, Switzerland) · 2022Article
- Building an extensible cell wall.Plant physiology · 2022Article
- Structure ofBiomacromolecules · 2022Article
- Polarized epithelium-sperm co-culture system reveals stimulatory factors for the secretion of mouse epididymal quiescin sulfhydryl oxidase 1.The Journal of reproduction and development · 2022Article
- Rice Brittle Culm19 Encoding Cellulose Synthase Subunit CESA4 Causes Dominant Brittle Phenotype But has No Distinct Influence on Growth and Grain Yield.Rice (New York, N.Y.) · 2021Article
- Phenotypic effects of changes in the FTVTxK region of anPlant direct · 2021Article
- Updating Insights into the Catalytic Domain Properties of PlantMolecules (Basel, Switzerland) · 2021Review
- Elucidating the Opportunities and Challenges for Nanocellulose Spinning.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- Structure ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Polysaccharide Biosynthesis: Glycosyltransferases and Their Complexes.Frontiers in plant science · 2021Article
- Cross-Sections of Nanocellulose from Wood Analyzed by Quantized Polydispersity of Elementary Microfibrils.ACS nano · 2020Article
- Biochemical and Genetic Analysis Identify CSLD3 as a beta-1,4-Glucan Synthase That Functions during Plant Cell Wall Synthesis.The Plant cell · 2020Article
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Authors and funding
13 authors at 1 institution in 1 country.
Venu Gopal VandavasiBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).ORCID http://orcid.org/0000-0002-8894-1395
Daniel K PutnamBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).ORCID http://orcid.org/0000-0001-8080-775X
Qiu ZhangBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Loukas PetridisBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
William T HellerBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).ORCID http://orcid.org/0000-0001-6456-2975
B Tracy NixonBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Candace H HaiglerBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Udaya KalluriBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Leighton CoatesBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Paul LanganBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).ORCID http://orcid.org/0000-0002-0247-3122
Jeremy C SmithBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Jens MeilerBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.).
Hugh O'NeillBiology and Soft Matter Division (V.G.V., Q.Z., W.T.H., L.C., H.O.), BioSciences Division (L.P., U.K.), Center for Molecular Biophysics (L.P., J.C.S.), and Neutron Sciences Directorate (P.L.), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831;Department of Biomedical Informatics (D.K.P., J.M.) and Department of Chemistry (J.M.), Vanderbilt University, Nashville, Tennessee 37232;Biochemistry and Molecular Biology, Pennsylvania State University, Pennsylvania 16802 (B.T.N.);Department of Crop Science and Department of Plant and Microbial Biology, North Carolina State University, North Carolina 27695 (C.H.H.); andDepartment of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996 (J.C.S., H.O.) oneillhm@ornl.gov.ORCID http://orcid.org/0000-0003-2966-5527
Oak Ridge National Laboratory · US
Funding
GPU-Accelerated Parallel Computer for Drug Discovery ApplicationsS10OD020154 · OD · VANDERBILT UNIVERSITY · PI SMITH, JARROD ANSON · 2015 to 2015
$225kAbstract
A cellulose synthesis complex with a "rosette" shape is responsible for synthesis of cellulose chains and their assembly into microfibrils within the cell walls of land plants and their charophyte algal progenitors. The number of cellulose synthase proteins in this large multisubunit transmembrane protein complex and the number of cellulose chains in a microfibril have been debated for many years. This work reports a low resolution structure of the catalytic domain of CESA1 from Arabidopsis (Arabidopsis thaliana; AtCESA1CatD) determined by small-angle scattering techniques and provides the first experimental evidence for the self-assembly of CESA into a stable trimer in solution. The catalytic domain was overexpressed in Escherichia coli, and using a two-step procedure, it was possible to isolate monomeric and trimeric forms of AtCESA1CatD. The conformation of monomeric and trimeric AtCESA1CatD proteins were studied using small-angle neutron scattering and small-angle x-ray scattering. A series of AtCESA1CatD trimer computational models were compared with the small-angle x-ray scattering trimer profile to explore the possible arrangement of the monomers in the trimers. Several candidate trimers were identified with monomers oriented such that the newly synthesized cellulose chains project toward the cell membrane. In these models, the class-specific region is found at the periphery of the complex, and the plant-conserved region forms the base of the trimer. This study strongly supports the "hexamer of trimers" model for the rosette cellulose synthesis complex that synthesizes an 18-chain cellulose microfibril as its fundamental product.
Indexed as
ArabidopsisArabidopsis ProteinsCatalytic DomainCelluloseEscherichia coliGlucosyltransferasesMicroscopy, Electron, TransmissionModels, MolecularMultiprotein ComplexesProtein MultimerizationProtein Structure, SecondaryRecombinant ProteinsScattering, Small AngleX-Ray DiffractionArabidopsis ProteinsCelluloseCESA1 protein, ArabidopsisGlucosyltransferasesMultiprotein ComplexesRecombinant Proteins
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