ArticleGenome biology2015
CRISPR/Cas9-mediated viral interference in plants.
Article in Genome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers.
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Who cites it
180 citing papers in PubMed, 497 citations in OpenAlex.
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- Evaluation of CRISPR/Cas9 based single and multiplex gRNAs against ChiLCV in tobacco.Journal, genetic engineering & biotechnology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Genome editing‑based strategies to combat geminiviruses: CRISPR/Cas9 and emerging high‑fidelity tools.Archives of microbiology · 2026Review
- Geminiviral-CR-gRNA expressed in cowpea efficiently edited MYMV and MYMIV genome to provide resistance against cowpea yellow mosaic disease without hampering plant growth and yield.BMC plant biology · 2026Article
- Carbon Dots and mesoporous silica nanocomposites improve spray-induced gene silencing to suppress plant RNA and DNA viruses.Scientific reports · 2026Article
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- Genome editing of susceptibility geneFrontiers in plant science · 2026Article
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- Review
- Review
- Strategies for plant-virus disease management from gene editing to nanotechnology.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Review
- Potential for Duplexed, In-Tandem gRNA-Mediated Suppression of Two Essential Genes of Tomato Leaf Curl New Delhi Virus in Crop Plants.Pathogens (Basel, Switzerland) · 2025Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Genomics in plant pathogen identification and control.Frontiers in plant science · 2025Review
- CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants.Plants (Basel, Switzerland) · 2024Review
- Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.International journal of molecular sciences · 2024Review
- Geminiviruses and Food Security: A Molecular Genetics Perspective for Sustainable Agriculture in Africa.Plants (Basel, Switzerland) · 2024Review
- Article
120 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species.
resultsIn this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses.
conclusionsThese data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.