ArticlePloS one2015
High-Resolution Melting Curve Analysis for Identification of Pasteurellaceae Species in Experimental Animal Facilities.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- High-resolution melting analysis in veterinary medicine: a systematic review of applications and diagnostic accuracy.Tropical animal health and production · 2025Pooled it
- Distinguishing fanged frogs (Limnonectes) species (Amphibia: Anura: Dicroglossidae), from Thailand using high resolution melting analysis.Scientific reports · 2023Article
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- RAPD and ERIC-PCR coupled with HRM for species identification of non-dysenteriae Shigella species; as a potential alternative method.BMC research notes · 2021Article
- Article
- Pitfalls and promises of raw drug identification techniques in the ayurvedic industry: an overview.3 Biotech · 2020Review
- Rapid identification of the Candida glabrata species complex by high-resolution melting curve analysis.Journal of clinical laboratory analysis · 2020Article
- High-resolution melting curve analysis: a novel method for identification of Mycoplasma species isolated from clinical cases of bovine and porcine respiratory disease.Tropical animal health and production · 2020Article
- Evaluation of a Novel Mitochondrial Pan-Journal of fungi (Basel, Switzerland) · 2019Article
- The Likelihood of Misidentifying Rodent Pasteurellaceae by Using Results from a Single PCR Assay.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice.BMC microbiology · 2019Article
- Resistin-like molecule β is a bactericidal protein that promotes spatial segregation of the microbiota and the colonic epithelium.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Exhaust Air Dust Monitoring is Superior to Soiled Bedding Sentinels for the Detection of Pasteurella pneumotropica in Individually Ventilated Cage Systems.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
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Authors and funding
3 authors.
Funding
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Abstract
Pasteurellaceae are among the most prevalent bacterial pathogens isolated from mice housed in experimental animal facilities. Reliable detection and differentiation of Pasteurellaceae are essential for high-quality health monitoring. In this study, we combined a real-time PCR assay amplifying a variable region in the 16S rRNA sequence with high-resolution melting curve analysis (HRM) to identify and differentiate among the commonly isolated species Pasteurella pneumotropica biotypes "Jawetz" and "Heyl", Actinobacillus muris, and Haemophilus influenzaemurium. We used a set of six reference strains for assay development, with the melting profiles of these strains clearly distinguishable due to DNA sequence variations in the amplicon. For evaluation, we used real-time PCR/HRM to test 25 unknown Pasteurellaceae isolates obtained from an external diagnostic laboratory and found the results to be consistent with those of partial 16S rRNA sequencing. The real-time PCR/HRM method provides a sensitive, rapid, and closed-tube approach for Pasteurellaceae species identification for health monitoring of laboratory mice.
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