ArticlePloS one2015
Genome-Wide Identification of New Reference Genes for qRT-PCR Normalization under High Temperature Stress in Rice Endosperm.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Article
- Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets.Plants (Basel, Switzerland) · 2023Article
- Cytological, transcriptome and miRNome temporal landscapes decode enhancement of rice grain size.BMC biology · 2023Article
- Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries.Plant methods · 2021Article
- Article
- Heat Stress Targeting Individual Organs Reveals the Central Role of Roots and Crowns in Rice Stress Responses.Frontiers in plant science · 2021Article
- Selection of Reference Genes for qRT-PCR Analysis in Medicinal PlantPlants (Basel, Switzerland) · 2020Article
- Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plants under differential biotic stresses.Scientific reports · 2020Article
- Identifying and validating housekeeping hybrid Prunus spp. genes for root gene-expression studies.PloS one · 2020Article
- Comprehensive molecular insights into the stress response dynamics of rice (Journal of biosciences · 2020Article
- Genome-wide identification of internal reference genes for normalization of gene expression values during endosperm development in wheat.Journal of applied genetics · 2019Article
- Stable reference genes for RT-qPCR analysis of gene expression in the Musa acuminata-Pseudocercospora musae interaction.Scientific reports · 2019Article
- Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus.Molecular biology reports · 2019Article
- Genes encoding membrane proteins showed stable expression in rice under aerobic condition: novel set of reference genes for expression studies.3 Biotech · 2018Article
- Suppression of OsMADS7 in rice endosperm stabilizes amylose content under high temperature stress.Plant biotechnology journal · 2018Article
- Sequencing the extrachromosomal circular mobilome reveals retrotransposon activity in plants.PLoS genetics · 2017Article
- Identification of Optimal Reference Genes for Expression Analysis in Radish (Frontiers in plant science · 2017Article
- Transcriptome analysis in different rice cultivars provides novel insights into desiccation and salinity stress responses.Scientific reports · 2016Article
- Suitable Reference Genes for Accurate Gene Expression Analysis in Parsley (Frontiers in plant science · 2016Article
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5 authors.
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Abstract
qRT-PCR is one of the most popular approaches to analyze specific gene expression level, and stably expressed reference genes are essential to obtain reliable results. However, many reference genes are only stable under certain circumstances and different reference genes might be required in different experiments. High temperature is a common stress that affects rice endosperm development and it has become a hot topic recently. Although study about reference genes at different developmental stages in rice has been reported, these genes may not be suitable to study high temperature mediated responses especially in endosperm. In our quest for proper reference genes to quantify gene expression in rice endosperm under high temperature, we studied 6 candidate genes selected from the transcriptome data and 11 housekeeping genes. All genes were analyzed with qRT-PCR and the expression stability was assessed with software geNorm and NormFinder. Fb15 and eIF-4a were identified as the two most stable genes in endosperm at different developmental stages, while high temperature treatment has a least effect on expression of Fb15 and UBQ5 in rice endosperm. Our results provide some good candidate reference genes for qRT-PCR normalization in rice endosperm under different temperatures.
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