ArticlePloS one2015
Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Sperm DNA fragmentation and its influence on mammalian reproduction.Nature reviews. Urology · 2026Review
- Exploring OR2H1-Mediated Sperm Chemotaxis: Development and Application of a Novel Microfluidic Device.Cells · 2025Article
- Microfluidic Sorting Can Be Applied for Assisted Reproduction Sperm Selection in Different Cases of Semen Abnormalities.Life (Basel, Switzerland) · 2025Article
- Advances in microfluidic technology for sperm screening and in vitro fertilization.Analytical and bioanalytical chemistry · 2024Review
- Visualization and Analysis of Mapping Knowledge Domain of Fluid Flow Related to Microfluidic Chip.ACS omega · 2024Article
- Article
- Lab-on-chip (LoC) application for quality sperm selection: An undelivered promise?Open research Europe · 2023Review
- Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device.Micromachines · 2022Article
- The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices.Journal of assisted reproduction and genetics · 2022Review
- Rheotaxis-based sperm separation using a biomimicry microfluidic device.Scientific reports · 2021Article
- Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia.Life (Basel, Switzerland) · 2021Article
- Visualization and Measurements of Blood Cells Flowing in Microfluidic Systems and Blood Rheology: A Personalized Medicine Perspective.Journal of personalized medicine · 2020Review
- Differences and Similarities: The Richness of Comparative Sperm Physiology.Physiology (Bethesda, Md.) · 2020Review
- Sperm Cell Driven Microrobots-Emerging Opportunities and Challenges for Biologically Inspired Robotic Design.Micromachines · 2020Review
- A high-throughput chemotaxis detection method for CCR4Journal of immunological methods · 2020Article
- Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa.Analytical chemistry · 2020Article
- Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract.Journal of visualized experiments : JoVE · 2019Article
- A Macroscopic Diffusion-Based Gradient Generator to Establish Concentration Gradients of Soluble Molecules Within Hydrogel Scaffolds for Cell Culture.Frontiers in chemistry · 2019Article
- Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species.Scientific reports · 2018Article
- Chemotactic behavior of spermatozoa captured using a microfluidic chip.Biomicrofluidics · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.