ArticleFungal genetics and biology : FG & B2016
Efficient engineering of marker-free synthetic allotetraploids of Saccharomyces.
Article in Fungal genetics and biology : FG & B, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Indirect Methods To Measure Unfolded Proteins In Living Cells Using Fluorescent Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- BeyondFrontiers in microbiology · 2022Article
- Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switching.Applied microbiology and biotechnology · 2021Article
- Hybridization ofMicroorganisms · 2021Article
- Industrially ApplicableApplied and environmental microbiology · 2021Article
- Lager Yeast Design Through Meiotic Segregation of aFrontiers in fungal biology · 2021Article
- Crossbreeding of Yeasts Domesticated for Fermentation: Infertility Challenges.International journal of molecular sciences · 2020Review
- Synthetic hybrids of six yeast species.Nature communications · 2020Article
- Does Inter-Organellar Proteostasis Impact Yeast Quality and Performance During Beer Fermentation?Frontiers in genetics · 2020Article
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Evolution of a novel chimeric maltotriose transporter in Saccharomyces eubayanus from parent proteins unable to perform this function.PLoS genetics · 2019Article
- Phenotype-Independent Isolation of InterspeciesFrontiers in microbiology · 2019Article
- Mitochondrial DNA and temperature tolerance in lager yeasts.Science advances · 2019Article
- Article
- Natural Variation in the Multidrug Efflux PumpGenetics · 2018Article
- A UniqueFrontiers in microbiology · 2018Article
- Improved cider fermentation performance and quality with newly generated Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids.Journal of industrial microbiology & biotechnology · 2017Article
- Novel brewing yeast hybrids: creation and application.Applied microbiology and biotechnology · 2017Review
- Hybridization and adaptive evolution of diverseBiotechnology for biofuels · 2017Article
- Directed Evolution Reveals Unexpected Epistatic Interactions That Alter Metabolic Regulation and Enable Anaerobic Xylose Use by Saccharomyces cerevisiae.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
Saccharomyces interspecies hybrids are critical biocatalysts in the fermented beverage industry, including in the production of lager beers, Belgian ales, ciders, and cold-fermented wines. Current methods for making synthetic interspecies hybrids are cumbersome and/or require genome modifications. We have developed a simple, robust, and efficient method for generating allotetraploid strains of prototrophic Saccharomyces without sporulation or nuclear genome manipulation. S. cerevisiae×S. eubayanus, S. cerevisiae×S. kudriavzevii, and S. cerevisiae×S. uvarum designer hybrid strains were created as synthetic lager, Belgian, and cider strains, respectively. The ploidy and hybrid nature of the strains were confirmed using flow cytometry and PCR-RFLP analysis, respectively. This method provides an efficient means for producing novel synthetic hybrids for beverage and biofuel production, as well as for constructing tetraploids to be used for basic research in evolutionary genetics and genome stability.
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