ArticleMicrobial cell factories2015
A new potential secretion pathway for recombinant proteins in Bacillus subtilis.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Overcoming cellular secretion bottlenecks: advanced secretion engineering and molecular tailoring for next-generation microbial α-amylases with enhanced industrial performance.World journal of microbiology & biotechnology · 2026Review
- Secretory expression of recombinant small laccase genes in Gram-positive bacteria.Microbial cell factories · 2023Article
- Secretome Analysis of the Plant Biostimulant Bacteria StrainsInternational journal of molecular sciences · 2022Article
- Design of a high-efficiency synthetic system for l-asparaginase production inEngineering in life sciences · 2019Article
- Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme.Microbial cell factories · 2018Article
- Conformational determinants necessary for secretion of Paecilomyces thermophila β-1,4-xylosidase that lacks a signal peptide.AMB Express · 2018Article
- The integrative effect of periphyton biofilm and tape grass (Vallisneria natans) on internal loading of shallow eutrophic lakes.Environmental science and pollution research international · 2018Article
- Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA.AMB Express · 2017Article
- Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.International journal of molecular sciences · 2017Article
- Use of a Novel Report Protein to Study the Secretion Signal of Flagellin in Bacillus subtilis.Current microbiology · 2016Article
- In-situ Adsorption-Biological Combined Technology Treating Sediment Phosphorus in all Fractions.Scientific reports · 2016Article
- A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis.Microbial cell factories · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSecretion of cytoplasmic expressed proteins into growth media has significant advantages. Due to the lack of an outer membrane, Bacillus subtilis is considered as a desirable 'cell factory' for the secretion of recombinant proteins. However, bottlenecks in the classical pathway for the secretion of recombinant proteins limit its use on a wide scale. In this study, we attempted to use four typical non-classically secreted proteins as signals to export three recombinant model proteins to the culture medium.
resultsAll four non-classically secreted proteins can direct the export of the intrinsically disordered nucleoskeletal-like protein (Nsp). Two of them can guide the secretion of alkaline phosphatase (PhoA). One can lead the secretion of the thermostable β-galactosidase BgaB, which cannot be secreted with the aid of typical Sec-dependent signal peptides.
conclusionOur results show that the non-classically secreted proteins lead the recombinant proteins to the culture medium, and thus non-classical protein secretion pathways can be exploited as a novel secretion pathway for recombinant proteins.
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