Evidence map›Paper›PMID 26555289›Full record

ArticleJournal of fluorescence2016

Synthesis and Evaluation of In Vitro DNA/Protein Binding Affinity, Antimicrobial, Antioxidant and Antitumor Activity of Mononuclear Ru(II) Mixed Polypyridyl Complexes.

Venkat Reddy Putta, Nagamani Chintakuntla, Rajender Reddy Mallepally, Srishailam Avudoddi, Nagasuryaprasad K, Deepika Nancherla, Yaswanth V V N, Prakasham R S, Satyanarayana Singh Surya, Satyanarayana Sirasani

Abstract read
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Article in Journal of fluorescence, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Ruthenium-based PACT agents based on bisquinoline chelates: synthesis, photochemistry, and cytotoxicity.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2021
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Venkat Reddy PuttaDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Nagamani ChintakuntlaDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Rajender Reddy MallepallyDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Srishailam AvudoddiDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Nagasuryaprasad KDepartment of Biochemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Deepika NancherlaDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Yaswanth V V NBioengineering and Environmental Sciences, CSIR-Indian Institute of Chemical Technology, Taranaka, Hyderabad, Telangana State, 500007, India.
Prakasham R SBioengineering and Environmental Sciences, CSIR-Indian Institute of Chemical Technology, Taranaka, Hyderabad, Telangana State, 500007, India.
Satyanarayana Singh SuryaDepartment of Biochemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India.
Satyanarayana SirasaniDepartment of Chemistry, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India. ssnsirasani@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The four novel Ru(II) complexes [Ru(phen)2MAFIP](2+) (1) [MAFIP = 2-(5-(methylacetate)furan-2-yl)-1 H-imidazo[4,5-f] [1, 10]phenanthroline, phen = 1,10-Phenanthroline], [Ru(bpy)2MAFIP](2+) (2) (bpy = 2,2'-bipyridine) and [Ru(dmb)2MAFIP](2+) (3) (dmb = 4,4'-dimethyl-2,2'-bipyridine) and [Ru(hdpa)2MAFIP](2+) (4) (hdpa = 2,2-dipyridylamine) have been synthesized and fully characterized via elemental analysis, NMR spectroscopy, EI-MS and FT-IR spectroscopy. In addition, the DNA-binding behaviors of the complexes 1-4 with calf thymus DNA were investigated by UV-Vis absorption, fluorescence studies and viscosity measurement. The DNA-binding experiments showed that the complexes 1-4 interact with CT-DNA through an intercalative mode. BSA protein binding affinity of synthesized complexes was determined by UV/Vis absorption and fluorescence emission titrations. The binding affinity of ruthenium complexes was supported by molecular docking. The photoactivated cleavage of plasmid pBR322 DNA by ruthenium complexes 1-4 was investigated. All the synthesized compounds were tested for antimicrobial activity by using three Gram-negative (Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa) and three Gram-positive (Micrococcus luteus, Bacillus subtilis and Bacillus megaterium) organisms, these results indicated that complex 3 was more activity compared to other complexes against all tested microbial strains while moderate antimicrobial activity profile was noticed for complex 4. The antioxidant activity experiments show that the complexes exhibit moderate antioxidant activity. The cytotoxicity of synthesized complexes on HeLa cell lines has been examined by MTT assay. The apoptosis assay was carried out with Acridine Orange (AO) staining methods and the results indicate that complexes can induce the apoptosis of HeLa cells. The cell cycle arrest investigated by flow cytometry and these results indicate that complexes 1-4 induce the cell cycle arrest at G0/G1 phase.

Indexed as

AnimalsAnti-Bacterial AgentsAntineoplastic AgentsAntioxidantsBinding SitesCattleCell Cycle CheckpointsCell ProliferationDNADose-Response Relationship, DrugDrug Screening Assays, AntitumorFluorescent DyesGram-Negative BacteriaGram-Positive BacteriaHeLa CellsHumansAnti-Bacterial AgentsAntineoplastic AgentsAntioxidantscalf thymus DNADNAFluorescent DyesOrganometallic CompoundsPhenanthrolinesRutheniumSerum Albumin, BovineAntimicrobial activityAntioxidantCytotoxicityDNA bindingRu(II) complexes

Identifiers

PMID26555289

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.