ArticleThe Journal of biological chemistry2015
Transmembrane Topologies of Ca2+-permeable Mechanosensitive Channels MCA1 and MCA2 in Arabidopsis thaliana.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall.International journal of molecular sciences · 2023Review
- Root osmotic sensing from local perception to systemic responses.Stress biology · 2022Review
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- MCAs in Arabidopsis are CaNature communications · 2021Article
- Between Stress and Response: Function and Localization of Mechanosensitive CaInternational journal of molecular sciences · 2021Review
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- PIEZO ion channel is required for root mechanotransduction inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls.Plants (Basel, Switzerland) · 2020Article
- CaScientific reports · 2018Article
- Post-translational processing and membrane translocation of the yeast regulatory Mid1 subunit of the Cch1/VGCC/NALCN cation channel family.The Journal of biological chemistry · 2017Article
- Plant mechanosensitive ion channels: an ocean of possibilities.Current opinion in plant biology · 2017Review
- Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) forms a CaScientific reports · 2016Article
- Calcium-Mediated Abiotic Stress Signaling in Roots.Frontiers in plant science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensing mechanical stresses, including touch, stretch, compression, and gravity, is crucial for growth and development in plants. A good mechanosensor candidate is the Ca(2+)-permeable mechanosensitive (MS) channel, the pore of which opens to permeate Ca(2+) in response to mechanical stresses. However, the structure-function relationships of plant MS channels are poorly understood. Arabidopsis MCA1 and MCA2 form a homotetramer and exhibit Ca(2+)-permeable MS channel activity; however, their structures have only been partially elucidated. The transmembrane topologies of these ion channels need to be determined in more detail to elucidate the underlying regulatory mechanisms. We herein determined the topologies of MCA1 and MCA2 using two independent methods, the Suc2C reporter and split-ubiquitin yeast two-hybrid methods, and found that both proteins are single-pass type I integral membrane proteins with extracellular N termini and intracellular C termini. These results imply that an EF hand-like motif, coiled-coil motif, and plac8 motif are all present in the cytoplasm. Thus, the activities of both channels can be regulated by intracellular Ca(2+) and protein interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.