ArticlePloS one2015
The Performance of the Four Anaerobic Blood Culture Bottles BacT/ALERT-FN, -FN Plus, BACTEC-Plus and -Lytic in Detection of Anaerobic Bacteria and Identification by Direct MALDI-TOF MS.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Comparative analytical performance of Autof MS2600 and Biotyper Sirius for rapid MALDI-TOF MS identification of microorganisms from positive blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Experimental study on factors influencing low identification rates and spectral quality ofMicrobiology spectrum · 2025Article
- Efficient protocol for isolating human fibroblast from primary skin cell cultures: application to keloid, hypertrophic scar, and normal skin biopsies.Biology methods & protocols · 2024Article
- A Dedicated Mycosis Flask Increases the Likelihood of Identifying Candidemia Sepsis.Journal of fungi (Basel, Switzerland) · 2023Article
- Considerations in evaluating equipment-free blood culture bottles: A short protocol for use in low-resource settings.PloS one · 2022Article
- Direct Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Testing from Positive Blood Cultures for Rapid Identification of Bloodstream Infection-Causing Anaerobic Bacteria.Journal of clinical microbiology · 2021Article
- Current Status of Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology.Molecules (Basel, Switzerland) · 2020Review
- Performance of a new combination of blood culture vials in sepsis detection: a 2-year retrospective comparison.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections.BMC infectious diseases · 2019Article
- Comparison of the Performance of Three Blood Culture Systems in a Chinese Tertiary-Care Hospital.Frontiers in cellular and infection microbiology · 2019Article
- Clinical Performance Evaluation of VersaTrek 528 Blood Culture System in a Chinese Tertiary Hospital.Frontiers in microbiology · 2018Article
- An Improved In-house MALDI-TOF MS Protocol for Direct Cost-Effective Identification of Pathogens from Blood Cultures.Frontiers in microbiology · 2017Article
- Individualized Approaches Are Needed for Optimized Blood Cultures.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Controlled Evaluation of the New BacT/Alert Virtuo Blood Culture System for Detection and Time to Detection of Bacteria and Yeasts.Journal of clinical microbiology · 2016Article
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5 authors.
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Abstract
Detection and identification of anaerobic bacteria in blood cultures (BC) is a well-recognized challenge in clinical microbiology. We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p<0.01) in the studied material. There was no significant difference in detection of anaerobic bacteria among the remaining bottle types. The 67 anaerobic bacteria that signalled positive in all four bottle types were analyzed to compare the time to detection (TTD) and isolates were directly identified by MALDI-TOF MS. There was a significant difference in TTD among the four bottle types (p<0.0001). The shortest median TTD was 18 h in BACTEC Lytic followed by BacT/ALERT FN (23.5 h), BACTEC Plus (27 h) and finally BacT/ALERT FN Plus (38 h) bottles. In contrast, MALDI-TOF MS performed similarly in all bottle types with accurate identification in 51/67 (76%) BacT/ALERT FN, 51/67 (76%) BacT/ALERT FN Plus, 53/67 (79%) BACTEC Plus and 50/67 (75%) BACTEC Lytic bottles. In conclusion, BACTEC Lytic bottles have significantly better detection rates and shorter TTD compared to the three other bottle types. The anaerobic BC bottles are equally suitable for direct MALDI-TOF MS for rapid and reliable identification of common anaerobic bacteria. Further clinical studies are warranted to investigate the performance of anaerobic BC bottles in detection of anaerobic bacteria and identification by direct MALDI-TOF MS.
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